7b84fe1d-42d0-439a-b60f-0b621f7ba93d http://ipt.gbif.pt/ipt/resource?r=ibi_mecoptera The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae) Daniel Oliveira CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Master’s Student
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
daniel.jet100@gmail.com 0000-0002-8733-4104
Sónia Ferreira CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 hiporame@gmail.com 0000-0002-6884-3966
Luís P. da Silva CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 lfpascoals@gmail.com 0000-0003-2358-1277
Pedro Brito Lopes CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
pedro.lopes275@gmail.com 0000-0002-5078-0602
Pedro Sousa CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 psousa@cibio.up.pt 0000-0002-5859-9656
Pedro Beja CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Coordinating Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 pbeja@cibio.up.pt 0000-0001-8164-0760
Daniel Oliveira CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Master’s Student
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
daniel.jet100@gmail.com 0000-0002-8733-4104
Sónia Ferreira CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 hiporame@gmail.com 0000-0002-6884-3966
Luís P. da Silva CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 lfpascoals@gmail.com 0000-0003-2358-1277
Pedro Brito Lopes CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
pedro.lopes275@gmail.com 0000-0002-5078-0602
Pedro Sousa CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 psousa@cibio.up.pt 0000-0002-5859-9656
Pedro Beja CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Coordinating Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 pbeja@cibio.up.pt 0000-0001-8164-0760
2020-08-03 eng The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases. Mecoptera n/a Panorpidae n/a Species n/a Occurrence GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type.xml Cytochrome c oxidase I (COI) n/a DNA barcode n/a Continental Portugal n/a Specimen GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License. http://www.boldsystems.org Continental Portugal -8.7 -6.4 42 39.8 2008-06-02 2019-06-28 This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal. kingdom Animalia phylum Arthropoda class Insecta order Mecoptera family Panorpidae The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. asNeeded Pedro Beja CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Coordinating Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 pbeja@cibio.up.pt 0000-0001-8164-0760
Sónia Ferreira CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Post-Doc Researcher
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 Vairão Porto 4485-661 PT
+351 252660411 hiporame@gmail.com 0000-0002-6884-3966
Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage. The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013). DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions. Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope. To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae) Pedro Beja 0000-0001-8164-0760 author Daniel Oliveira 0000-0002-8733-4104 author Luís P. da Silva 0000-0003-2358-1277 author Pedro Brito Lopes 0000-0002-5078-0602 author Sónia Ferreira 0000-0002-6884-3966 author Pedro Sousa 0000-0002-5859-9656 originator The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest. InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250. Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts. The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search.
2020-05-18T01:10:48.443+01:00 dataset Oliveira D, Ferreira S, P. da Silva L, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae). v1.7. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/rmj9sz Monserrat, V. J. and Tillier, P. (2013) ‘Sinopsis de los Mecópteros de la Península Ibérica (Insecta, Mecoptera)’, Boletín de la Sociedad Entomológica Aragonesa (S.E.A.), 53(1926), pp. 143–152. Ward, P. H. (1983) ‘Scorpion-flies of the panorpa cognata-complex in the western palaearctic region (Mecoptera)’, Journal of Natural History, 17(4), pp. 627–645. doi: 10.1080/00222938300770521. https://cibio.up.pt/upload/image/logotipos/2-logo.png CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 InBIO Barcoding Initiative alcohol Voucher tube 1 13 DNA extractions 1 13 7b84fe1d-42d0-439a-b60f-0b621f7ba93d/v1.8.xml