The InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera)

Hugo Rebelo1, Sónia Ferreira1, Francisco Amorim1, Pedro Horta1, Helena Raposeira1, Helena Santos1, Pedro Beja1, Pedro Sousa1, Vanessa Mata1

1 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal

Corresponding author(s): Hugo Rebelo (hugo.rebelo@cibio.up.pt)Sónia Ferreira (hiporame@gmail.com)Francisco Amorim (famorim@cibio.up.pt)Pedro Horta (pedrhorta27@hotmail.com)Helena Raposeira (helena.raposeira@gmail.com)Helena Santos (helena.vianasantos@gmail.com)Pedro Beja (pbeja@cibio.up.pt)Pedro Sousa (psousa@cibio.up.pt)Vanessa Mata (vanessamata@cibio.up.pt)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Rebelo H, Ferreira S, Amorim F, Horta P, Raposeira H, Santos H, Beja P, Sousa P, Mata V (2021): The InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera). v1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=ibi_chiroptera&v=1.4

Abstract

The dataset contains 63 records of Chiroptera species collected in continental Portugal and was published by Rebelo et al (2020)". Twenty-six bat species currently known for continental Portugal are represented including the first record of Myotis alcathoe for the country. Bat samples were collected under the scope of several projects spanning from 2005 to 2018. All bats were captured during mist-netting sessions or using harp-traps at roost exits. A non-lethal 3mm wing punch was collected from several individuals and stored in 96% ethanol from where DNA was extracted. The DNA barcoding of these samples was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. DNA extractions are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Keywords: Chiroptera, Species, Occurrence, COI, DNA barcode, Continental Portugal, Occurrence, Occurrence

General description

Purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Project details

Project title: The InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera)
Personnel: Hugo Rebelo, Sónia Ferreira, Francisco Amorim, Pedro Horta, Helena Raposeira, Helena Santos, Pedro Beja, Pedro Sousa, Vanessa Mata
Funding: The work was supported by the EnvMetaGen project, funded by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 668981, and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).
Study area descriptions/descriptor: Samples were collected in 11 continental Portugal districts from a total of 31 different localities. These localities encompassed a great variety of habitats. Sampling was conducted from 2005 to 2018, although most specimens (76.2%) were collected in the years 2013 and 2016.
Design description: Specimens in this dataset were collected in field work carried out between 2005 and 2018. Specimens resulted from field work directed specifically for the sampling of Chiroptera using mist-nets or harp-traps at roost exits.

Data published through GBIF: http://ipt.gbif.pt/ipt/resource?r=ibi_chiroptera

Taxonomic coverage

General taxonomic coverage description: This dataset is composed entirely of data relating to 63 Chiroptera records. Sixty-three specimens were determined to species level.
Overall, 26 species are represented in the dataset. These species belong to three families, the majority of which belong to the Vespertilionidae (21 species or 80.8%), with additional representatives from Rhinolophidae (4 species) and a single species in the Molossidae. Vespertilionidae also accounts for almost ninety percent (88.9%) of all collected samples, Rhinolophidae (9.5%) and a single sample was collected from the Molossidae family.
Taxonomic ranks
Kingdom: Animalia
Phylum: Chordata
Class: Mammalia
Order: Chiroptera
Family: Molossidae, Rhinolophidae, Vespertilionidae

Spatial coverage

General spatial coverage: Continental Portugal
Coordinates: 37°23'60''N and 41°53'60''N Latitude; 9°18'0''W and 6°54'0''W Longitude 

Temporal coverage: June 27, 2005 - August 22, 2018

Natural collections description

Collection name: InBIO Barcoding Initiative
Collection identifier: 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Curatorial unit: Between 1 and 63 (DNA extractions)

Methods

Method step description: Samples were collected from bats captured using mist-nets or harp-traps at roost exits and identified morphologically by experts. A non-lethal 3mm wing punch was collected each individual and stored in 96% ethanol from where DNA was extracted and COI DNA barcode fragment was sequenced. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Study extent description: Samples were collected in 31 different sites of continental Portugal. Sampling was conducted from 2005 to 2018. Most specimens (76.2%) were collected in the years 2013 and 2016.
Sampling description: Specimens resulted from field work directed specifically for the sampling of Chiroptera using mist-nets or harp-traps at roost exits.
Quality control description: To each sample the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.

Datasets

Dataset description
Object name: Darwin Core Archive The InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera)
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://ipt.gbif.pt/ipt/archive.do?r=ibi_chiroptera
Publication date of data: 2021-01-05
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2019-10-29
Hierarchy level: Dataset

References
Rebelo, H., Ferreira, S., Amorim, F., Horta, P., Raposeira, H., Santos, H., Beja, P. & Mata, V.A. (2020). Hidden in our pockets: building of a DNA barcode library unveils the first record of Myotis alcathoe for Portugal. Biodiversity Data Journal, 8: e54479. https://doi.org/10.3897/BDJ.8.e54479 https://doi.org/10.3897/BDJ.8.e54479