The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera)

Sónia Ferreira1, Daniel Oliveira1, Pedro Brito Lopes1, Pedro Sousa1, Pedro Beja1, Sónia Ferreira2

1 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal; 2 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, , Portugal

Corresponding author(s): Sónia Ferreira (hiporame@gmail.com)Daniel Oliveira (daniel.jet100@gmail.com)Pedro Brito Lopes (pedro.lopes275@gmail.com)Pedro Sousa (psousa@cibio.up.pt)Pedro Beja (pbeja@cibio.up.pt), Sónia Ferreira (hiporame@gmail.com)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Ferreira S, Oliveira D, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera). v1.5. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/jups46

Abstract

The dataset contains 56 records of Earwigs (Dermaptera) species collected from 2006 to 2019 in continental Portugal. All captured specimens were identified to species level, belonging to ten of the fourteen species of Dermaptera present in Portugal. 
Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the within the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases. 

Keywords: Dermaptera, Earwig, Species, Occurrence, Cytochrome c oxidase I (COI), DNA barcode, Continental Portugal, Specimen

General description

Purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Project details

Project title: The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera)
Personnel: Pedro Beja, Daniel Oliveira, Pedro Brito Lopes, Sónia Ferreira, Pedro Sousa
Funding: InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Study area descriptions/descriptor: Specimens were captured during several field incursions throughout Portugal from 2006 to 2019 in 30 localities, distributed across 11 districts.
Design description: The specimens in this dataset were collected in field work incursion carried out between 2006 and 2019, encompassing several sites throughout Portugal in an attempt to have a good representation of the country’s fauna. Specimens of Dermaptera were detected by direct search of the vegetation and soil.

Data published through GBIF: http://ipt.gbif.pt/ipt/resource?r=ibi_dermaptera

Taxonomic coverage

General taxonomic coverage description: This dataset is composed of data relating to 56 Dermaptera specimens belonging to ten species present in Portugal. The four families of Dermaptera known to occur in Portugal are represented in the dataset, with all species of Spongiphoridae and Labiduridae known from the country fully represented. The most quantitatively represented species is Forficula auricularia with fifteen records.
Taxonomic ranks
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Dermaptera
Family: Anisolabididae, Forficulidae, Labiduridae, Spongiphoridae

Spatial coverage

General spatial coverage: Continental Portugal.
Coordinates: 37°36'0''N and 41°42'0''N Latitude; 9°18'0''W and 6°17'60''W Longitude 

Temporal coverage: July 21, 2006 - May 30, 2019

Natural collections description

Parent collection identifier: CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Collection name: InBIO Barcoding Initiative
Collection identifier: 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Specimen preservation method: Alcohol
Curatorial unit: Between 1 and 56 (Voucher tube)
Curatorial unit: Between 1 and 56 (DNA extractions)

Methods

Method step description: 
- . 	Specimens of Dermaptera were captured in continental Portugal from 2006 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage. 
- . 	The specimens were morphologically identified resorting to available literature (Chopard, 1922, 1943; Lapeira and Pascual, 1980; Albouy and Caussanel, 1990; Bivar de Sousa, 1997). 
- . 	Six specimens from each species (or all specimens, when less than six available) were selected for DNA extraction and sequencing, based on geographical location. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced. 
- . 	All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine. 

- . 	Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Study extent description: Specimens of Dermaptera were captured in thirty sites, distributed across eleven districts of the country. Sampling was conducted from 2006 to 2019, in several field work expeditions.
Sampling description: Specimens were captured from 2006 to 2019 in continental Portugal by direct search of thirty sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.
Quality control description: To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Datasets

Dataset description
Object name: Darwin Core Archive The InBIO Barcoding Initiative Database: Portuguese Earwigs (Dermaptera)
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://ipt.gbif.pt/ipt/archive.do?r=ibi_dermaptera
Publication date of data: 2020-07-08
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2020-05-18
Hierarchy level: Dataset

References
Albouy, V. and Caussanel, C. (1990) Dermaptères ou Perc-Oreilles, Faune de France. Federation Francaise des Societes de Sciences Naturelles.
Bivar de Sousa, A. (1997) ‘Dermápteros (Insecta: Dermaptera) novos ou pouco conhecidos para Portugal continental’, Boletim da Sociedade Portuguesa de Entomologia, 167(VI–17), pp. 229–244.
Chopard, L. (1922) 3. Orthoptéres et Dermaptères, Faune de France. Paris: Office Central de Faunistique.
Chopard, L. (1943) I. Orthoptèroïdes de l’Afreique du Nord, Faune de L’Empire Français. Paris.
Lapeira, A. and Pascual, F. (1980) ‘Estudio preliminar de los Dermápteros de la Fauna Ibérica’, Trabajo Monografíco del Departamento de Zoología de la Universidad de Granada, 3(4), pp. 53–99.