The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)

Sónia Ferreira1, Luís P. da Silva1, Pedro Sousa1, Pedro Beja1

1 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal

Corresponding author(s): Sónia Ferreira (hiporame@gmail.com)Luís P. da Silva (lfpascoals@gmail.com)Pedro Sousa (psousa@cibio.up.pt)Pedro Beja (pbeja@cibio.up.pt)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Ferreira S, P. da Silva L, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae). v1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/7559ru

Abstract

The dataset contains 11 records of Stag beetle (Lucanidae) species collected from 2015 to 2019 in continental Portugal. All specimens were identified to species level, and belong to 3 species of the Lucanidae (Coleoptera). Specimens were detected during fieldwork  by direct search and stored in 96% ethanol. A tissue sample (a leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences available were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Keywords: Coleoptera, Lucanidae, Species, Cytochrome c oxidase I (COI), DNA barcode, Continental Portugal, Occurrence, Specimen

General description

Purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Project details

Project title: The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)
Personnel: Pedro Beja, Pedro Sousa, Luís P. da Silva, Sónia Ferreira
Funding: InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Study area descriptions/descriptor: Specimens were collected in five Portugal districts and a total of ten sites were sampled. Sampling was conducted from 2015 to 2019.
Design description: Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens were located by direct searches carried out during field work, which enabled the sampling of both live and dead specimens.

Data published through GBIF: http://ipt.gbif.pt/ipt/resource?r=ibi_lucanidae

Taxonomic coverage

General taxonomic coverage description: This dataset is composed of data relating to 11 Lucanidae (Coleoptera) specimens. All specimens were determined to species level. Three of the four Stag beetle species known to occur in continental Portugal are represented in the dataset, with Dorcus parallelipipedus as the most represented species in the dataset, accounting for seven of the eleven specimens.
Taxonomic ranks
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Coleoptera
Family: Lucanidae

Spatial coverage

General spatial coverage: Continental Portugal
Coordinates: 37°0'0''N and 41°53'60''N Latitude; 9°0'0''W and 6°42'0''W Longitude 

Temporal coverage: June 17, 2015 - September 19, 2019

Natural collections description

Parent collection identifier: CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Collection name: InBIO Barcoding Initiative
Collection identifier: 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Specimen preservation method: Alcohol
Curatorial unit: Between 1 and 11 (Voucher tube)
Curatorial unit: Between 1 and 11 (DNA extractions)

Methods

Method step description: Sampling was conducted in continental Portugal from 2015 and 2019, and consisted in direct search. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed from which DNA was extracted and the COI DNA barcode fragment was sequenced. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Study extent description: Specimens were collected in ten localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions.
Sampling description: Specimens were collected in ten localities of continental Portugal. Sampling was conducted between 2015 and 2019, by direct search. Collected specimens were identified using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis.
Quality control description: To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.

Datasets

Dataset description
Object name: Darwin Core Archive The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae)
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://ipt.gbif.pt/ipt/archive.do?r=ibi_lucanidae
Publication date of data: 2020-06-25
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2020-01-23
Hierarchy level: Dataset