The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01

Daniel Oliveira1, Martin Corley2, Luís P. da Silva1, Vanessa Alves Mata1, Pedro Brito Lopes1, Pedro Sousa1, Pedro Beja1, Sónia Ferreira1, Martin Corley3, Pedro Sousa4

1 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal; 2 Pucketty Farm Cottage, SN7 8JP, Faringdon, United Kingdom; 3 Pucketty Farm Cottage, SN7 8JP, Faringdon , United Kingdom; 4 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão

Corresponding author(s): Daniel Oliveira (daniel.jet100@gmail.com)Martin Corley (martin.corley@btinternet.com)Luís P. da Silva (lfpascoals@gmail.com)Vanessa Alves Mata (vanessamata@cibio.up.pt)Pedro Brito Lopes (pedro.lopes275@gmail.com)Pedro Sousa (psousa@cibio.up.pt)Pedro Beja (pbeja@cibio.up.pt)Sónia Ferreira (hiporame@gmail.com), Martin Corley (martin.corley@btinternet.com), Pedro Sousa (psousa@cibio.up.pt)

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Oliveira, D., Corley, M., da Silva, L.P., Mata, V.A., Lopes, P.B., Sousa, P., Beja, P. & & Ferreira, S. (2021). The InBIO Barcoding Initiative Database: Lacewings (Neuroptera). v1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/6cyrxd

Abstract

The dataset contains 234 records of Lacewings (Neuroptera) species collected from 2006 to 2019 in continental Portugal (Oliveira et al., 2021). Specimens were detected and captured by direct search of the environment and by using both UV and mercury-vapor lamps to attract the insects. Captured specimens were preserved in 96% ethanol. All captured specimens were identified to species level. Samples of each species were selected for DNA sequencing based on their geographic provenance. From each specimen, one leg (tissue sample) was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Keywords: Neuroptera, Lacewings, Species, Occurrence, Cytochrome c oxidase I (COI), DNA barcode, Continental Portugal, Specimen

General description

Purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Project details

Project title: The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01
Personnel: Pedro Beja, Sónia Ferreira, Daniel Oliveira, Martin Corley, Luís P. da Silva, Vanessa Alves Mata, Pedro Brito Lopes, Pedro Sousa
Funding: InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Study area descriptions/descriptor: Specimens were collected in 12 continental Portugal administrative districts. Eighty-seven different localities were sampled. Sampling was conducted from 2006 to 2019, although most specimens (87.6%) were collected in the period between 2017 to 2019.
Design description: The specimens in this dataset were collected in fieldwork incursions carried out between 2006 and 2019, encompassing several sites throughout continental Portugal in an attempt to have a good representation of the country’s Neuroptera fauna. Specimens were morphologically identified and DNA barcoded.

Data published through GBIF: http://ipt.gbif.pt/ipt/resource?r=ibi_neuroptera_01

Taxonomic coverage

General taxonomic coverage description: This dataset is composed of data relating to 234 Neuroptera specimens belonging to 50 species, that represent half of the species known from continental Portugal. These species are representative of nine of the ten families of Neuroptera known from continental Portugal. The families with the highest number of recorded specimens and species diversity are the Chrysopidae (87 records, 21 species), Myrmeleontidae (68 records, 12 species) and Hemerobiidae (42 records, 8 species).
Taxonomic ranks
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Neuroptera
Family: Ascalaphidae, Berothidae, Chrysopidae, Coniopterygidae, Dilaridae, Hemerobiidae, Mantispidae, Myrmeleontidae, Nemopteridae

Spatial coverage

General spatial coverage: Continental Portugal
Coordinates: 37°10'12''N and 41°58'48''N Latitude; 9°27'36''W and 6°18'36''W Longitude 

Temporal coverage: June 30, 2006 - September 20, 2019

Natural collections description

Parent collection identifier: CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Collection name: InBIO Barcoding Initiative
Collection identifier: 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Specimen preservation method: Alcohol
Curatorial unit: Between 1 and 234 (Voucher tube)
Curatorial unit: Between 1 and 234 (DNA extractions)

Methods

Method step description: 
- . 	Specimens were collected in 87 different localities of continental Portugal. Fieldwork was carried out between 2006 and 2019, with 87.6% of the records obtained between 2017 and 2019.
- . 	Specimens were collected by direct search of the sampling site while also using both UV LED and mercury-vapor lamps as lures distributed along the site. Specimens were preserved in 96% alcohol and deposited in the IBI reference collection.
- . 	Specimens were morphologically identified using a stereoscopic microscope, an Olympus SZX2-ILLT, and resorting to most recent literature on Iberian Neuroptera (Monserrat, 2014a, 2014b, 2014c, 2016a, 2016b; Monserrat and Acevedo, 2013; Monserrat et al., 2012a; 2012b, 2014)
- . 	All specimens were DNA barcoded. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All tissue samples and DNA extracts were deposited in the IBI collection.
- . 	All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- . 	Prior to GBIF submission, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).
Study extent description: Specimens were collected in 87 different localities of continental Portugal. Sampling was conducted from 2006 to 2019, in several fieldwork expeditions. Most specimens (87.6%) were collected in the period between 2017 to 2019.
Sampling description: The studied material was collected in 87 different Portuguese localities. Sampling was conducted between 2006 and 2019. Specimens were captured by direct search of the environment and by using both UV LED and mercury-vapor lamps to attract the insects. Specimens were preserved in 96% ethanol for downstream molecular analysis and stored at the InBIO Barcoding Initiative (IBI )reference collection.
Quality control description: To each specimen, the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine.
Prior to GBIF submission, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Datasets

Dataset description
Object name: Darwin Core Archive The InBIO Barcoding Initiative Database: Lacewings (Neuroptera) 01
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://ipt.gbif.pt/ipt/archive.do?r=ibi_neuroptera_01
Publication date of data: 2021-08-06
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2021-04-19
Hierarchy level: Dataset

References
Monserrat, V. J. 2014a. Los berótidos de la Península Ibérica (Insecta: Neuropterida: Neuroptera: Berothidae). Heteropterus Revista de Entomología, 14(1): 31–54.
Monserrat, V. J. 2014b. Los diláridos de la Península Ibérica (Insecta: Neuropterida: Neuroptera: Dilaridae). Heteropterus Revista de Entomología, 14(2): 187–214.
Monserrat, V. J. 2014c. Revisión de los mantíspidos de la Península Ibérica y Baleares (Insecta, Neuropterida, Neuroptera, Mantispidae). Graellsia, 70(2): e012. https://doi.org/10.3989/graellsia.2014.v70.115 
Monserrat, V. J. 2016a. Los coniopterígidos de la Península Ibérica e Islas Baleares (Insecta Neuropterida, Neuroptera: Coniopterygidae). Graellsia, 72(2): e047. https://doi.org/10.3989/graellsia.2016.v72.157
Monserrat, V. J. 2016b. Los crisópidos de la Península Ibérica y Baleares (Insecta, Neuropterida, Neuroptera: Chrysopidae). Graellsia, 72(1): e037. https://doi.org/10.3989/graellsia.2016.v72.143
Monserrat, V. J. and Acevedo, F. 2013. Los mirmeleóntidos (hormigas-león) de la Península Ibérica e Islas Baleares (Insecta, Neuropterida, Neuroptera, Myrmeleontidae). Graellsia, 69(2): 283-321. https://doi.org/10.3989/graellsia.2013.v69.098
Monserrat, V. J., Acevedo, F. & Triviño, V. 2012. Los ascaláfidos de la Península Ibérica y Baleares (Insecta : Neuroptera: Ascalaphidae). Heteropterus Revista de Entomología, 12(1): 33–58.
Monserrat, V. J., Badano, D. & Acevedo, F. 2014. Nuevos datos de ascaláfidos para la Península Ibérica, con una nueva especie para la fauna europea (Insecta: Neuropterida: Neuroptera: Ascalaphidae). Heteropterus Revista de Entomología, 14(2): 147–167.
Monserrat, V. J., Triviño, V.  & Acevedo, F. 2012. Los nemoptéridos y crócidos de la Península Ibérica (Insecta: Neuroptera: Nemopteridae, Crocidae). Heteropterus Revista de Entomología, 12(2): 231–255.
Oliveira, D., Chaves, C., Pinto, J., Paupério, J., Fonseca, N., Beja, P. & Ferreira, S. 2021. DNA Barcoding of Portuguese Lacewings (Neuroptera) and Snakeflies (Raphidioptera) (Insecta, Neuropterida). ZooKeys, 1054: 67-84. https://doi.org/10.3897/zookeys.1054.64608