The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01

Luis Martín1, Sónia´ Ferreira2, Jesús Martínez1, Marcus A. González1, Martin Corley3, José Manuel Grosso-Silva4, Lorenzo Quaglietta2, Pedro Sousa2, Pedro Beja2, Sónia Ferreira2, Martin Corley5, Pedro Sousa6

1 Department Zoología, Antropología Física & Genética, Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain; 2 CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7, 4485-661, Vairão, Portugal; 3 Pucketty Farm Cottage, SN7 8JP, Faringdon, United Kingdom; 4 Museu de História Natural e da Ciência da Universidade do Porto, Porto, Portugal; 5 ; 6 

Corresponding author(s): Luis MartínSónia´ Ferreira (hiporame@gmail.com)Jesús MartínezMarcus A. GonzálezMartin Corley (martin.corley@btinternet.com)José Manuel Grosso-Silva (jmgrossosilva@gmail.com)Lorenzo Quaglietta (lorenzo.quaglietta@cibio.up.pt)Pedro Sousa (psousa@cibio.up.pt)Pedro Beja (pbeja@cibio.up.pt), Martin Corley

Received {date}; Revised {date}; Accepted {date}; Published {date}

Citation: Combination of authors, year of data paper publication (in parentheses), Title, Journal Name, Volume, Issue number (in parentheses), and doi of the data paper.

Resource Citation

Martín L, Ferreira S, Martínez J, González M A, Corley M, Grosso-Silva J M, Quaglietta L, Sousa P, Beja P (2022): The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01. v1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=ibi_trichoptera_01&amp;v=1.1

Abstract

The dataset contains 438 records of Trichoptera species from 22 of the 23 families known from the Iberian Peninsula. Specimens were collected between 1975 to 2018 in Portugal, Spain and France (Paupério et al., 2023). Specimens have been identified to species or subspecies level, in a total of 141 species  representing 37% of the Caddisflies known for the Iberian Peninsula. Specimens were captured during fieldwork directed specifically for the sampling of Trichoptera using different methodologies and stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. Sequencing of the 658 bp COI DNA barcode was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases. Specimens are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain). All DNA extracts are deposited in the IBI collection.

Keywords: Occurrence, Specimen, Trichoptera, Caddisflies, Occurrence records, Species distributions, Iberian Peninsula, Portugal, Spain, France, DNA barcode, Cytochrome c oxidase I (COI)

General description

Purpose: The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

Project details

Project title: The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01
Personnel: Luis Martín, Sónia Ferreira, Jesús Martínez, Marcus A. González, Martin Corley, José Manuel Grosso-Silva, Lorenzo Quaglietta, Pedro Sousa, Pedro Beja
Funding: The InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). SF was supported by an individual research contract (2020.03526.CEECIND) funded by FCT.
Study area descriptions/descriptor: Specimens were collected in nine continental Portugal districts, 16 Spanish provinces and one French department. One hundred and two different localities were sampled. Collection occurred between 1975 and 2018, although most specimens were collected in 2015 (25.4%), 2016 (17.8%) and 2017 (19.5%).
Design description: Specimens in this dataset were collected in field work carried out between 1975 and 2018, directed specifically for the sampling of Trichoptera using different methodologies. Specimens were morphologically identified and DNA barcoded.

Data published through GBIF: http://ipt.gbif.pt/ipt/resource?r=ibi_trichoptera_01

Taxonomic coverage

General taxonomic coverage description: This dataset is composed of data relating to 438 Trichoptera specimens. All specimens were determined to species level except for two specimens that were identified to genus level. Overall, 141 species are represented in the dataset. Four species have been further identified to subspecies level: Wormaldia variegata mattheyi Schmid, 1952, Philopotamus montanus caurelensis González & Terra, 1979, Wormaldia variegata mattheyi Schmid, 1952, Rhyacophila dorsalis albarracina Malicky, 2002 and Rhyacophila mocsaryi tredosensis Schmid, 1952.
The species represented in the dataset belong to 56 genera unevenly distributed, with the families Limnephilidae (11 genera, 19.6% of the total), Leptoceridae (8 genera, 14.3%), Glossosomatidae, Hydroptilidae and Psychomyiidae (each with 4 genera or 7.1% of the total) accounting for 55% of the genera represented in the dataset. Three families account for 43% of the species represented in the dataset, Limnephilidae and Rhyacophilidae (each with 23 species or 16.3% of the total) and Hydropsychidae (15 species, 10.6%).
Taxonomic ranks
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Trichoptera Family:
Family: Apataniidae, Beraeidae, Brachycentridae, Calamoceratidae, Ecnomidae, Glossosomatidae, Goeridae, Helicopsychidae, Hydropsychidae, Hydroptilidae, Lepidostomatidae, Leptoceridae, Limnephilidae, Odontoceridae, Philopotamidae, Phryganeidae, Polycentropodidae, Psychomyiidae, Ptilocolepidae, Rhyacophilidae, Sericostomatidae, Uenoidae

Spatial coverage

General spatial coverage: Iberian Peninsula, France
Coordinates: 37°30'7.2''N and 43°15'36''N Latitude; 8°56'38.4''W and 0°13'44.4''W Longitude 

Temporal coverage: May 3, 1975 - May 16, 2018

Natural collections description

Collection name: InBIO Barcoding Initiative
Collection identifier: 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Specimen preservation method: Alcohol
Curatorial unit: Between 1 and 438 (Voucher tube)
Curatorial unit: Between 1 and 438 (DNA extractions)

Methods

Method step description: 
- . 	Specimens were collected in 142 different localities in the Iberian Peninsula and France. Field work was carried out between 1975 and 2018, with 62% of the records made between the years 2015 and 2017.
- . 	Specimens were collected during fieldwork by direct search and individual netting of specimens, by sweeping the vegetation or were directly collected at light traps (using both UV and mercury vapor lights) and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).
- . 	All specimens were morphologically identified using the available literature.
- . 	DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All DNA extracts were deposited in the IBI collection.
- . 	All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- . 	Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).
Study extent description: Portugal, Spain and France.
Sampling description: The studied material was collected in 142 different localities, 74 in Spain, 66 in Portugal and two in France. Sampling was conducted between 1975 and 2018, although 62% of the records were made between 2015 and 2017. Specimens were collected by direct search and individual netting of specimens, by sweeping the vegetation with a net or were directly collected at light traps, using both UV and mercury vapor lights. Captured specimens deposited in the IBI reference collection were preserved in 96% alcohol.
Quality control description: For all DNA barcoded specimens, their morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Datasets

Dataset description
Object name: Darwin Core Archive The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01
Character encoding: UTF-8
Format name: Darwin Core Archive format
Format version: 1.0
Distribution: http://ipt.gbif.pt/ipt/archive.do?r=ibi_trichoptera_01
Publication date of data: 2023-01-19
Language: English
Licences of use: Creative Commons Attribution (CC-BY) 4.0 License

Metadata language: English
Date of metadata creation: 2022-06-13
Hierarchy level: Dataset

References
Pauperio, J., Gonzalez, L.M., Martinez, J., González, M.A., Martins, F.M., Veríssimo, J., Puppo, P., Pinto. J., Chaves, C., Pinho, C.J., Grosso-Silva, J.M., Quaglietta, L., Silva, T.L.L., Sousa, P., Alves, P.C., Fonseca, N., Beja, P. & Ferreira, S. (2023). The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot. Biodiversity Data Journal 11: e97484.
https://doi.org/10.3897/BDJ.11.e97484