說明
The dataset contains 412 records of Diptera species from the families Limoniidae, Pediciidae and Tipulidae, collected between 2003 to 2019 in Portugal, including the Azores and Madeira archipelagos (Ferreira et al., 2021; Oosterbroek et al., 2020; Starý, 2014). Specimens have been identified to species or subspecies level, in a total of 83 species representing 58% of the Craneflies known for Portugal. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and the majority was stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by the EnvMetaGen and PORBIOTA projects. The dataset “DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae” is part of a group of Diptera datasets published by IBI, the first of which, “The InBIO Barcoding Initiative Database: Diptera 01” (https://doi.org/10.15468/q1bvt3) has already been made available through GBIF. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank databases. All DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 412 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Ferreira, S., Oosterbroek, P., Starý, J., Mata, V.A., Silva, L.P., Sousa, P., Beja, P. (2021): The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_02&v=1.6
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 66a5be5f-2471-4c38-b966-c3f9a8794832。 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。
關鍵字
Diptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Portugal; Azores; Madeira; Craneflies; Occurrence
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
http://orcid.org/0000-0002-6884-3966
- 元數據提供者
- 元數據提供者 ●
- 出處
- 元數據提供者 ●
- 出處
- 元數據提供者
- 元數據提供者 ●
- 出處 ●
- 連絡人
- 元數據提供者
- 元數據提供者
地理涵蓋範圍
Continental Portugal, Autonomous Regions of the Azores and of Madeira.
| 界定座標範圍 | 緯度南界 經度西界 [32.65, -25.51], 緯度北界 經度東界 [41.97, -6.34] |
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分類群涵蓋範圍
This dataset is composed of data relating to 412 Diptera specimens. All specimens were determined to species level except for seven specimens. Overall, 83 species are represented in the dataset. Two species have been further identified to subspecies level, Nephrotoma suturalis wulpiana (Bergroth, 1888) and Nephrotoma appendiculata pertenua Oosterbroek, 1978. These species belong to 31 genera unevenly distributed among three Craneflies families, Limoniidae (21 genera), Tipulidae (4 genera) and Pediciidae (2 genera). The families Limoniidae and Tipulidae account for a similar number of collected specimens, 197 and 211, respectively. However, Limoniidae has a higher proportion of recorded species, 45, when compared with Tipulidae, with 36 species.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Diptera |
| Superfamily | Tipuloidea |
| Family | Limoniidae, Pediciidae, Tipulidae |
時間涵蓋範圍
| 起始日期 / 結束日期 | 2003-09-08 / 2019-10-14 |
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計畫資料
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| 計畫名稱 | The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae |
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| 辨識碼 | InBIO Barcoding Initiative |
| 經費來源 | The InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). LPdS and SF were supported by individual research contracts (CEECIND/02064/2017, 2020.03526.CEECIND) funded by FCT. JS was funded by the Ministry of Culture of the Czech Republic through institutional financing of long-term conceptual development of the Silesian Museum research institution (MK000100595). |
| 研究區域描述 | Specimens were collected in 14 continental Portugal administrative districts and in the Azores and Madeira archipelagos. Eighty-three different localities were sampled. Collection occurred between 2003 and 2019, although most specimens were collected in 2018 (32%) and 2019 (50%). |
| 研究設計描述 | Specimens in this dataset were collected in field work carried out between 2003 and 2019, directed specifically for the sampling of Diptera using different methodologies. Specimens were morphologically identified and DNA barcoded. |
參與計畫的人員:
- 作者
- 作者
取樣方法
The studied material was collected in 83 different Portuguese localities. Sampling was conducted between 2003 and 2019, although 82% of the records were made in the years 2018 and 2019. Specimens were collected by direct search and individual netting of specimens, by sweeping the vegetation with a net or were directly collected at light traps, using both UV and mercury vapor lights. Captured specimens deposited in the IBI reference collection were preserved in 96% alcohol.
| 研究範圍 | Portugal, including the Autonomous Regions of the Azores and of Madeira. |
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| 品質控管 | For all DNA barcoded specimens, their morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/). |
方法步驟描述:
- Specimens were collected in 83 different Portuguese localities. Field work was carried out between 2003 and 2019, with 82% of the records made in the years 2018 and 2019.
- Specimens were collected during fieldwork by direct search and individual netting of specimens, by sweeping the vegetation or were directly collected at light traps (using both UV and mercury vapor lights) and preserved in 96% alcohol. The majority of captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All specimens were morphologically identified using the available literature except seven that were identified using the BOLD Identification Engine . For some specimens it was necessary to prepare and then exam their terminalia.
- All specimens were DNA barcoded. For these, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All DNA extracts were deposited in the IBI collection.
- All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).
收藏資料
| 蒐藏名稱 | InBIO Barcoding Initiative |
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| 蒐藏編號 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 標本保存方法 | Alcohol |
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| 管理單位 | 在...之間 1 和 381 Voucher tube , 在...之間 1 和 412 DNA extractions |
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引用文獻
- Ferreira, S., Oosterbroek, P., Starý, J., Sousa, P., Mata, V.A., da Silva, L.P., Paupério, J., Beja, P. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 02 - Limoniidae, Pediciidae and Tipulidae. Biodiversity Data Journal 9: e69841. https://doi.org/10.3897/BDJ.9.e69841
- Oosterbroek, P., Starý, J., Andrade, R., Hancock, E.G. & Ferreira, S. (2020). The Craneflies of continental Portugal (Diptera, Limoniidae, Pediciidae, Tipulidae) including 28 species new for Portugal. Boletín de la Asociación española de Entomología 44 (3-4): 317-358.
- Starý, J. (2014). Some records of Limoniidae and Pediciidae (Diptera) from Portugal and Spain. Acta Musei Silesiae, Scientiae Naturales, 63(1): 83-95. https://doi.org/10.2478/cszma-2014-0010
額外的詮釋資料
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
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| 替代的識別碼 | http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_02 |