The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 438 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Martín L, Ferreira S, Martínez J, González M A, Corley M, Grosso-Silva J M, Quaglietta L, Sousa P, Beja P (2022): The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Trichoptera 01. v1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=ibi_trichoptera_01&v=1.1

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: f2e21a20-4637-4b9a-81f7-8d5e3426a7ce.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.

Keywords

Occurrence; Specimen; Trichoptera; Caddisflies; Occurrence records; Species distributions; Iberian Peninsula; Portugal; Spain; France; DNA barcode; Cytochrome c oxidase I (COI)

Contacts

Geographic Coverage

Iberian Peninsula, France

Taxonomic Coverage

This dataset is composed of data relating to 438 Trichoptera specimens. All specimens were determined to species level except for two specimens that were identified to genus level. Overall, 141 species are represented in the dataset. Four species have been further identified to subspecies level: Wormaldia variegata mattheyi Schmid, 1952, Philopotamus montanus caurelensis González & Terra, 1979, Wormaldia variegata mattheyi Schmid, 1952, Rhyacophila dorsalis albarracina Malicky, 2002 and Rhyacophila mocsaryi tredosensis Schmid, 1952. The species represented in the dataset belong to 56 genera unevenly distributed, with the families Limnephilidae (11 genera, 19.6% of the total), Leptoceridae (8 genera, 14.3%), Glossosomatidae, Hydroptilidae and Psychomyiidae (each with 4 genera or 7.1% of the total) accounting for 55% of the genera represented in the dataset. Three families account for 43% of the species represented in the dataset, Limnephilidae and Rhyacophilidae (each with 23 species or 16.3% of the total) and Hydropsychidae (15 species, 10.6%).

Temporal Coverage

Project Data

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

The personnel involved in the project:

Sampling Methods

The studied material was collected in 142 different localities, 74 in Spain, 66 in Portugal and two in France. Sampling was conducted between 1975 and 2018, although 62% of the records were made between 2015 and 2017. Specimens were collected by direct search and individual netting of specimens, by sweeping the vegetation with a net or were directly collected at light traps, using both UV and mercury vapor lights. Captured specimens deposited in the IBI reference collection were preserved in 96% alcohol.

Method step description:

1. Specimens were collected in 142 different localities in the Iberian Peninsula and France. Field work was carried out between 1975 and 2018, with 62% of the records made between the years 2015 and 2017.
2. Specimens were collected during fieldwork by direct search and individual netting of specimens, by sweeping the vegetation or were directly collected at light traps (using both UV and mercury vapor lights) and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).
3. All specimens were morphologically identified using the available literature.
4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. All DNA extracts were deposited in the IBI collection.
5. All sequences in the dataset were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.4 (http://openrefine.org/).

Collection Data

Bibliographic Citations

1. Pauperio, J., Gonzalez, L.M., Martinez, J., González, M.A., Martins, F.M., Veríssimo, J., Puppo, P., Pinto. J., Chaves, C., Pinho, C.J., Grosso-Silva, J.M., Quaglietta, L., Silva, T.L.L., Sousa, P., Alves, P.C., Fonseca, N., Beja, P. & Ferreira, S. (2023). The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot. Biodiversity Data Journal 11: e97484. https://doi.org/10.3897/BDJ.11.e97484

Additional Metadata