MONICEPH - Monitoring cephalopods during whale watching activity in the Azores

資料紀錄

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亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

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如何引用

研究者應依照以下指示引用此資源。:

Suciu S, Azevedo J M (2025). MONICEPH - Monitoring cephalopods during whale watching activity in the Azores. Version 1.5. Universidade dos Açores. Occurrence dataset. http://ipt.gbif.pt/ipt/resource?r=moniceph&v=1.5

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Universidade dos Açores。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: c268bcb5-2d16-4796-b47b-7ea01a7a06b7。  Universidade dos Açores 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。

關鍵字

cephalopoda; deep-ocean; cetaceans; citizen science; azores

聯絡資訊

地理涵蓋範圍

Archipelago of the Azores

分類群涵蓋範圍

oceanic cephalopods

時間涵蓋範圍

計畫資料

MONICEPH project stores and analyses samples of cephalopods collected at the sea surface of the Azores, mostly during touristic activities. Remains of cephalopods are believed to be brought to the surface by cetaceans. The data collected helps increase understanding of oceanic cephalopod ecology and cetacean diets. Started in 2019, the dataset was compiled in collaboration with whale-watching companies on the islands of São Miguel, Faial, Pico and Terceira, and received additional samples from other sources. Project coordination by Stéphanie R.A. Suciu and José M.N. Azevedo.

參與計畫的人員:

取樣方法

A total of 14 whale watching companies participated. Each company received a boxed sampling kit, and the staff got trained in sampling and observations as described in Suciu et al, 2021. Every time cephalopod remains were observed, a sample was taken and preserved in 96% ethanol in supplied jars. Additionally, the collectors recorded, as a minimum, the time and geographic position, and information about the species of cetaceans in the area and their behavior. When possible, photographs of the remains were also taken. Upon return to the land base, the samples were stored at 4°C in the fridge. The samples were collected under the following permits: AMP/2020/014 from the Direção Regional dos Assuntos do Mar (DRAM) in in 2020-21; AMP/2022/007 from the DRAM and 20/2022/DRCTD from the Direção Regional da Ciência e Transição Digital (DRCT) in 2022; in LMAS-DRPM/2023/ 07 from the DIREÇÃO REGIONAL DE POLÍTICAS MARÍTIMAS (DRPM) and CCIR-RAA/2023/36 from the Direção Regional da Ciência, Inovação e Desenvolvimento (DRCT) in 2023; and LMAS-DRPM/2024/10 from the DRPM and CCIR-RAA/2024/45 from the DRCT in 2024.

方法步驟描述:

1. DNA extraction, PCR amplification and Sanger sequencing The DNA extraction was performed with the E.Z.N.A.® Mollusc DNA Kit (Omega Bio-tek, Georgia, USA) according to manufacturer’s protocol. The two primers HCO2198 and LCO1490 were used to PCR amplify a partial 710-bp fragment from the mitochondrial COI gene using the conditions in Folmer et al, 1994. The amplicons generated were individually Sanger sequenced with BigdyeTerminator 3.1, then run on ABI 3730XL (Applied Biosystems, Foster City, CA, USA), using the forward PCR primer. Forward sequences were cleaned using Geneious (v. 2024.0.7, Biomatters, Auckland, NZ), then were compared to the NCBI nucleotide database using the BLAST algorithm for taxonomic assignment. Morphological Determination When possible, on-site observations or collected images were analysed to determine species based on morphological characteristics using the Cephalopod identification keys from Gomes-Pereira et al., 2016.

收藏資料

引用文獻

1. Gomes-Pereira, J & Gonçalves, João & Clarke, M & Gomes-Pereira, Jose. (2016). Cephalopod identification keys to Histioteuthidae, Cranchiidae and Octopodiformes of the Azores, with an updated check-list.
2. Suciu, S.R.A., J. Zereba, L. Fiori and J.M.N. Azevedo 2021. Whale-watching contributions for the study of cetacean-cephalopod interactions. Arquipelago. Life and Marine Sciences 38: 1 – 6. https://doi.org/10.25752/arq.26535
3. Folmer O, Black M, Hoeh W, et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–370 https://doi.org/10.1371/journal.pone.0013102

額外的詮釋資料