Description
The dataset contains 71 records of Plecoptera species collected from 2004 to 2018 in the Iberian Peninsula. All specimens have been morphologically identified to species level, and belong to 29 species in total. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 71 records.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Please be aware, this is an old version of the dataset. Researchers should cite this work as follows:
Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=plecoptera_01&v=1.3
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 50942caf-a62e-44c7-9998-e2a949aa85b2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.
Keywords
Plecoptera; Occurrence records; Species distributions; Continental Portugal; Continental Spain; DNA barcode; Cytochrome c oxidase I (COI); Occurrence; Specimen
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Geographic Coverage
Iberian Peninsula
| Bounding Coordinates | South West [36.617, -7.749], North East [42.506, 3.036] |
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Taxonomic Coverage
This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Plecoptera |
| Family | Capniidae, Chloroperlidae, Leuctridae, Nemouridae, Perlidae, Perlodidae, Taeniopterygidae |
Temporal Coverage
| Start Date / End Date | 2004-06-22 / 2018-05-19 |
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Project Data
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Title | The InBIO Barcoding Initiative Database: Plecoptera 01 |
|---|---|
| Identifier | InBIO Barcoding Initiative |
| Funding | This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017. |
| Study Area Description | Iberian Peninsula. |
| Design Description | Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded. |
The personnel involved in the project:
Sampling Methods
The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).
| Study Extent | Iberian Peninsula. |
|---|---|
| Quality Control | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org). |
Method step description:
- Specimens were collected in 40 different localities of Iberian Peninsula. Sampling was conducted from 2004 to 2018, and consisted of direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed, from which DNA was extracted and the COI DNA barcode fragment was sequenced. Data generated were submitted to BOLD, GenBank and GBIF.
Collection Data
| Collection Name | InBIO Barcoding Initiative |
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| Collection Identifier | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Specimen preservation methods | Alcohol |
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| Curatorial Units | Between 1 and 71 Voucher tube , Between 1 and 71 DNA extractions |
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Bibliographic Citations
- Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp.
- Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571. Accessed on: 2020-4-09. http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571
Additional Metadata
| Purpose | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
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| Alternative Identifiers | http://ipt.gbif.pt/ipt/resource?r=plecoptera_01 |