Occurrence

The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera

Version 1.3 published by CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal on 29 July 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 71 records in English (14 KB)  - Update frequency: as needed
Metadata as an EML file download in English (27 KB)
Metadata as an RTF file download in English (15 KB)

Description

The dataset contains 71 records of Plecoptera species collected from 2004 to 2018 in the Iberian Peninsula. All specimens have been morphologically identified to species level, and belong to 29 species in total. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 71 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Please be aware, this is an old version of the dataset.  Researchers should cite this work as follows:

Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. http://ipt.gbif.pt/ipt/resource?r=plecoptera_01&v=1.3

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 50942caf-a62e-44c7-9998-e2a949aa85b2.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.

Keywords

Plecoptera; Occurrence records; Species distributions; Continental Portugal; Continental Spain; DNA barcode; Cytochrome c oxidase I (COI); Occurrence; Specimen

Contacts

Sónia Ferreira
  • Metadata Provider
  • Originator
  • Point Of Contact
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
+351 252660411
J. Manuel Tierno de Figueroa
  • Metadata Provider
  • Originator
Full Professor
Universidad de Granada
ES
Filipa M S Martins
  • Metadata Provider
  • Originator
PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Joana Veríssimo
  • Metadata Provider
  • Originator
PhD student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Lorenzo Quaglietta
  • Metadata Provider
  • Originator
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
José M. Grosso-Silva
  • Metadata Provider
  • Originator
Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
PT
Pedro Brito Lopes
  • Metadata Provider
  • Originator
Researcher
Rua do Torgal, nº 16 (Trigais)
Covilhã
PT
Pedro Sousa
  • Metadata Provider
  • Originator
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
+351 252660411
Pedro Beja
  • Metadata Provider
  • Originator
  • Point Of Contact
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
+351 252660411

Geographic Coverage

Iberian Peninsula

Bounding Coordinates South West [36.617, -7.749], North East [42.506, 3.036]

Taxonomic Coverage

This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.

Kingdom Animalia
Phylum Arthropoda
Class Insecta
Order Plecoptera
Family Capniidae, Chloroperlidae, Leuctridae, Nemouridae, Perlidae, Perlodidae, Taeniopterygidae

Temporal Coverage

Start Date / End Date 2004-06-22 / 2018-05-19

Project Data

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Title The InBIO Barcoding Initiative Database: Plecoptera 01
Identifier InBIO Barcoding Initiative
Funding This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017.
Study Area Description Iberian Peninsula.
Design Description Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded.

The personnel involved in the project:

Sampling Methods

The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).

Study Extent Iberian Peninsula.
Quality Control All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org).

Method step description:

  1. Specimens were collected in 40 different localities of Iberian Peninsula. Sampling was conducted from 2004 to 2018, and consisted of direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed, from which DNA was extracted and the COI DNA barcode fragment was sequenced. Data generated were submitted to BOLD, GenBank and GBIF.

Collection Data

Collection Name InBIO Barcoding Initiative
Collection Identifier 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Specimen preservation methods Alcohol
Curatorial Units Between 1 and 71 Voucher tube ,  Between 1 and 71 DNA extractions

Bibliographic Citations

  1. Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp.
  2. Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571. Accessed on: 2020-4-09. http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571

Additional Metadata

Purpose The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Alternative Identifiers http://ipt.gbif.pt/ipt/resource?r=plecoptera_01