Occurrence

The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea)

Dernière version Publié par CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal le 8 juillet 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
The dataset contains 18 records of Stick insects (Phasmatodea) species collected from 2006 to 2019 in continental Portugal. All captured specimens were identified to species level, belonging to the two species of Phasmatodea present in Portugal. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted ... plus

Description

The dataset contains 18 records of Stick insects (Phasmatodea) species collected from 2006 to 2019 in continental Portugal. All captured specimens were identified to species level, belonging to the two species of Phasmatodea present in Portugal. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 18 enregistrements.

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Téléchargements

Téléchargez la dernière version de la ressource en tant qu'Archive Darwin Core (DwC-A), ou les métadonnées de la ressource au format EML ou RTF :

Données sous forme de fichier DwC-A (zip) télécharger 18 enregistrements dans Anglais (8 kB) - Fréquence de mise à jour: quand cela est nécessaire
Métadonnées sous forme de fichier EML télécharger dans Anglais (22 kB)
Métadonnées sous forme de fichier RTF télécharger dans Anglais (17 kB)

Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea). v1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/wppjdk

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 9c95b714-d716-408e-a893-860254c0a706.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.

Mots-clé

Phasmatodea; Stick insect; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen

Contacts

Personne ayant créé cette ressource:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Personne pouvant répondre aux questions sur la ressource:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Personne ayant renseigné les métadonnées:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Sousa
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Couverture géographique

Continental Portugal.

Enveloppe géographique Sud Ouest [38, -8,9], Nord Est [42, -6,9]

Couverture taxonomique

This dataset is composed of data relating to 18 Phasmatodea specimens belonging to the two species present in Portugal, Leptynia attenuata and Clonopsis gallica. These species belong to two different families, Diapheromeridae and Bacillidae, respectively.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Phasmatodea
Family  Diapheromeridae,  Bacillidae

Couverture temporelle

Date de début / Date de fin 2006-07-22 / 2019-05-26

Données sur le projet

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Titre The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea)
Identifiant InBIO Barcoding Initiative
Financement InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Description du domaine d'étude / de recherche Specimens were captured throughout Portugal from 2006 to 2019 in ten different localities distributed across five districts.
Description du design The specimens in this dataset were collected in field work incursions carried out between 2006 and 2019, encompassing several sites throughout Portugal. Specimens of Phasmatodea were detected by direct search of vegetation.

Les personnes impliquées dans le projet:

Méthodes d'échantillonnage

Specimens were captured from 2006 to 2019 by direct search of ten sampling sites in continental Portugal. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection.

Etendue de l'étude Specimens of Phasmatodea were captured in ten sites distributed across the country, despite a concentration of records in the NE regions. Sampling was conducted from 2006 to 2019, in several field work expeditions.
Contrôle qualité To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Description des étapes de la méthode:

  1. Specimens of Phasmatodea were captured in continental Portugal from 2006 and 2019 by direct search of vegetation. Collected specimens were preserved in 96% ethanol prior to storage.
  2. The specimens were morphologically identified resorting to available literature (Scali, Milani and Passamonti, 2012, 2013).
  3. Six specimens from each species (or all specimens, when less than six available) were selected for DNA extraction and sequencing, based on geographical location. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  4. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  5. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Données de collection

Nom de la collection InBIO Barcoding Initiative
Identifiant de collection 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Identifiant de la collection parente CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Méthode de conservation des spécimens Alcohol
Unités de conservation Entre (nombre minimal) 1 et (nombre maximal) 18 Voucher tube ,  Entre (nombre minimal) 1 et (nombre maximal) 18 DNA extractions

Citations bibliographiques

  1. Scali, V., Milani, L. and Passamonti, M. (2012) ‘Revision of the stick insect genus Leptynia: Description of new taxa, speciation mechanism and phylogeography’, Contributions to Zoology, 81(1), pp. 25–42. doi: 10.1163/18759866-08101002. doi: 10.1163/18759866-08101002.
  2. Scali, V., Milani, L. and Passamonti, M. (2013) ‘Description and ecology of new Pijnackeria stick insects: Four bisexual species and a triploid parthenogen with their phyletic relationships’, Journal of Zoological Systematics and Evolutionary Research, 51(3), pp. 213–226. doi: 10.1111/jzs.12018. doi: 10.1111/jzs.12018.

Métadonnées additionnelles

Objet The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Identifiants alternatifs 9c95b714-d716-408e-a893-860254c0a706
http://ipt.gbif.pt/ipt/resource?r=ibi_phasmatodea