Описание
The dataset contains 18 records of Stick insects (Phasmatodea) species collected from 2006 to 2019 in continental Portugal. All captured specimens were identified to species level, belonging to the two species of Phasmatodea present in Portugal. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
Записи данных
Данные этого occurrence ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 18 записей.
Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.
Версии
В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.
Как оформить ссылку
Исследователи должны дать ссылку на эту работу следующим образом:
Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea). v1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/wppjdk
Права
Исследователи должны соблюдать следующие права:
Публикующей организацией и владельцем прав на данную работу является CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).
Регистрация в GBIF
Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 9c95b714-d716-408e-a893-860254c0a706. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Portugal.
Ключевые слова
Phasmatodea; Stick insect; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
Контакты
- Metadata Provider ●
- Originator
http://orcid.org/0000-0002-8733-4104
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Metadata Provider
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Metadata Provider
Географический охват
Continental Portugal.
| Ограничивающие координаты | Юг Запад [38, -8,9], Север Восток [42, -6,9] |
|---|
Таксономический охват
This dataset is composed of data relating to 18 Phasmatodea specimens belonging to the two species present in Portugal, Leptynia attenuata and Clonopsis gallica. These species belong to two different families, Diapheromeridae and Bacillidae, respectively.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Phasmatodea |
| Family | Diapheromeridae, Bacillidae |
Временной охват
| Дата начала / Дата окончания | 2006-07-22 / 2019-05-26 |
|---|
Данные проекта
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Название | The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea) |
|---|---|
| Идентификатор | InBIO Barcoding Initiative |
| Финансирование | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Описание района исследования | Specimens were captured throughout Portugal from 2006 to 2019 in ten different localities distributed across five districts. |
| Описание плана выполнения исследований | The specimens in this dataset were collected in field work incursions carried out between 2006 and 2019, encompassing several sites throughout Portugal. Specimens of Phasmatodea were detected by direct search of vegetation. |
Исполнители проекта:
Методы сбора
Specimens were captured from 2006 to 2019 by direct search of ten sampling sites in continental Portugal. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection.
| Охват исследования | Specimens of Phasmatodea were captured in ten sites distributed across the country, despite a concentration of records in the NE regions. Sampling was conducted from 2006 to 2019, in several field work expeditions. |
|---|---|
| Контроль качества | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). |
Описание этапа методики:
- Specimens of Phasmatodea were captured in continental Portugal from 2006 and 2019 by direct search of vegetation. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Scali, Milani and Passamonti, 2012, 2013).
- Six specimens from each species (or all specimens, when less than six available) were selected for DNA extraction and sequencing, based on geographical location. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Данные коллекции
| Название коллекции | InBIO Barcoding Initiative |
|---|---|
| Идентификатор коллекции | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Идентификатор родительской коллекции | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Методы сохранения образцов | Alcohol |
|---|
| Единицы хранения | Между 1 и 18 Voucher tube , Между 1 и 18 DNA extractions |
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Библиографические ссылки
- Scali, V., Milani, L. and Passamonti, M. (2012) ‘Revision of the stick insect genus Leptynia: Description of new taxa, speciation mechanism and phylogeography’, Contributions to Zoology, 81(1), pp. 25–42. doi: 10.1163/18759866-08101002. doi: 10.1163/18759866-08101002.
- Scali, V., Milani, L. and Passamonti, M. (2013) ‘Description and ecology of new Pijnackeria stick insects: Four bisexual species and a triploid parthenogen with their phyletic relationships’, Journal of Zoological Systematics and Evolutionary Research, 51(3), pp. 213–226. doi: 10.1111/jzs.12018. doi: 10.1111/jzs.12018.
Дополнительные метаданные
| Цель | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Альтернативные идентификаторы | 9c95b714-d716-408e-a893-860254c0a706 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_phasmatodea |