Description
The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 71 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. v1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/3vkdr2
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 50942caf-a62e-44c7-9998-e2a949aa85b2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.
Mots-clé
Plecoptera; Occurrence records; Species distributions; Continental Portugal; Continental Spain; DNA barcode; Cytochrome c oxidase I (COI); Occurrence; Specimen; Occurrence
Contacts
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http://orcid.org/0000-0002-6884-3966
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Couverture géographique
Iberian Peninsula
| Enveloppe géographique | Sud Ouest [36,617, -7,749], Nord Est [42,506, 3,036] |
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Couverture taxonomique
This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Plecoptera |
| Family | Capniidae, Chloroperlidae, Leuctridae, Nemouridae, Perlidae, Perlodidae, Taeniopterygidae |
Couverture temporelle
| Date de début / Date de fin | 2004-06-22 / 2018-05-19 |
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Données sur le projet
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Titre | The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera |
|---|---|
| Identifiant | InBIO Barcoding Initiative |
| Financement | This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017. |
| Description du domaine d'étude / de recherche | Iberian Peninsula. |
| Description du design | Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded. |
Les personnes impliquées dans le projet:
Méthodes d'échantillonnage
The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).
| Etendue de l'étude | Iberian Peninsula. |
|---|---|
| Contrôle qualité | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org). |
Description des étapes de la méthode:
- Specimens were collected in 40 different localities of the Iberian Peninsula. Fieldwork was carried out from 2004 to 2018.
- Specimens were collected during fieldwork by direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Collected specimens were stored in 96% ethanol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Data was submitted to GBIF, after being checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Données de collection
| Nom de la collection | InBIO Barcoding Initiative |
|---|---|
| Identifiant de collection | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Méthode de conservation des spécimens | Alcohol |
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| Unités de conservation | Entre (nombre minimal) 1 et (nombre maximal) 71 Voucher tube , Entre (nombre minimal) 1 et (nombre maximal) 71 DNA extractions |
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Citations bibliographiques
- Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp.
- Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571. Accessed on: 2020-4-09.
- Ferreira, S., Tierno de Figueroa, J.M., Martins, F.M., Verissimo, J., Quaglietta, L., Grosso-Silva, J.M., Lopes, P.B., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Biodiversity Data Journal, 8: e55137. https://doi.org/10.3897/BDJ.8.e55137
Métadonnées additionnelles
| Objet | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identifiants alternatifs | 50942caf-a62e-44c7-9998-e2a949aa85b2 |
| http://ipt.gbif.pt/ipt/resource?r=plecoptera_01 |