The dataset contains 71 records of Plecoptera specimens collected from 2004 to 2018 in the Iberian Peninsula (Ferreira et al., 2020). Twenty-nine stoneflies species are represented in the dataset, contributing to the knowledge on the DNA barcodes and distribution of the Plecoptera in Iberia. Specimens were captured during fieldwork directed specifically for the sampling of Plecoptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 71 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Baixe a última versão do recurso de dados, como um Darwin Core Archive (DwC-A) ou recurso de metadados, como EML ou RTF:
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Pesquisadores deveriam citar esta obra da seguinte maneira:
Ferreira S, Tierno de Figueroa J M, Martins F M S, Veríssimo J, Quaglietta L, Grosso-Silva J M, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. v1.4. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/3vkdr2
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 50942caf-a62e-44c7-9998-e2a949aa85b2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Portugal.
Plecoptera; Occurrence records; Species distributions; Continental Portugal; Continental Spain; DNA barcode; Cytochrome c oxidase I (COI); Occurrence; Specimen; Occurrence
Quem criou esse recurso:
Quem pode responder a perguntas sobre o recurso:
Quem preencher os metadados:
|Coordenadas delimitadoras||Sul Oeste [36,617, -7,749], Norte Leste [42,506, 3,036]|
This dataset is composed of data relating to 71 Plecoptera specimens. All specimens were determined to species level. Overall, 29 species are represented in the dataset. These species belong to 16 genera and 7 families.
|Família||Capniidae, Chloroperlidae, Leuctridae, Nemouridae, Perlidae, Perlodidae, Taeniopterygidae|
|Data Inicial / Data final||2004-06-22 / 2018-05-19|
Dados Sobre o Projeto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
|Título||The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera|
|Identificador||InBIO Barcoding Initiative|
|Financiamento||This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA— Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER- 022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of the research conducted at the Long Term Research Site of Baixo Sabor, (LTER_EU_PT_002). FMSM was supported by FCT PhD grant SFRH/BD/104703/2014, and JV by FCT PhD grant SFRH/BD/133159 /2017.|
|Descrição da Área de Estudo||Iberian Peninsula.|
|Descrição do Design||Specimens were collected in field work carried out between 2004 and 2018. They were morphologically identified and DNA barcoded.|
O pessoal envolvido no projeto:
Métodos de Amostragem
The studied material was collected in 40 different localities from Iberian Peninsula. Sampling was conducted between 2004 and 2018 on a wide range of habitats, using mainly hand-held sweep-nets or direct search for specimens. Collected specimens were examined in ethanol using a binocular stereoscopic microscope, and they were stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed using keys and descriptions from literature (mainly Tierno de Figueroa et al. 2003 , Tierno de Figueroa et al. 2015).
|Área de Estudo||Iberian Peninsula.|
|Controle de Qualidade||All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org).|
Descrição dos passos do método:
- Specimens were collected in 40 different localities of the Iberian Peninsula. Fieldwork was carried out from 2004 to 2018.
- Specimens were collected during fieldwork by direct search of specimens on rocks and vegetation of streams and river margins, and in the use of entomological nets to intercept specimens flight. Collected specimens were stored in 96% ethanol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Data was submitted to GBIF, after being checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Dados de Coleção
|Nome da Coleção||InBIO Barcoding Initiative|
|Identificador da Coleção||4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970|
|Métodos de preservação do espécime||Álcool|
|Unidades de Curadoria||Entre 1 e 71 Voucher tube , Entre 1 e 71 DNA extractions|
- Tierno de Figueroa J, Sánchez-Ortega A, Membiela-Iglesia P, Luzón-Ortega J (2003). Plecoptera. Fauna Ibérica. CSIC, Madrid, 404 pp.
- Tierno de Figueroa J, Luzón-Ortega J, López-Rodríguez M (2015) Orden Plecoptera. In: IBERFAUNA. El Banco de Datos de la Fauna Ibérica. Museo Nacional de Ciencias Naturales (CSIC). http://iberfauna.mncn.csic.es/showficha.aspx?rank=J&idtax=571. Accessed on: 2020-4-09.
- Ferreira, S., Tierno de Figueroa, J.M., Martins, F.M., Verissimo, J., Quaglietta, L., Grosso-Silva, J.M., Lopes, P.B., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of Iberian Plecoptera. Biodiversity Data Journal, 8: e55137. https://doi.org/10.3897/BDJ.8.e55137
|Propósito||The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.|