Description
The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 135 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Sousa, P., Grosso-Silva, J. M., Andrade, R., Beja, P., Ferreira, S. (2021): The InBIO Barcoding Initiative Database: Hemiptera 01. v1.2 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/8hn25y
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 963a25a6-839c-4bff-9889-97fc8385f564. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.
Mots-clé
Hemiptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contacts
- Fournisseur Des Métadonnées ●
- Créateur
http://orcid.org/0000-0002-5859-9656
- Fournisseur Des Métadonnées ●
- Créateur
- Fournisseur Des Métadonnées ●
- Créateur
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
Couverture géographique
Continental Portugal.
| Enveloppe géographique | Sud Ouest [37,257, -9,465], Nord Est [41,979, -6,344] |
|---|
Couverture taxonomique
This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Hemiptera |
| Family | Acanthosomatidae, Alydidae, Aphalaridae, Aphrophoridae, Aradidae, Auchenorrhyncha, Berytidae, Cercopidae, Cicadellidae, Cicadidae, Coreidae, Corixidae, Cydnidae, Delphacidae, Dictyopharidae, Flatidae, Gerridae, Heteroptera, Hydrometridae, Lygaeidae, Membracidae, Miridae, Nabidae, Nepidae, Notonectidae, Pentatomidae, Potamocoridae, Pyrrhocoridae, Reduviidae, Rhopalidae, Rhyparochromidae, Stenocephalidae, Thaumastocoridae |
Couverture temporelle
| Date de début / Date de fin | 2015-03-16 / 2019-09-20 |
|---|
Données sur le projet
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Titre | The InBIO Barcoding Initiative Database: Hemiptera 01 |
|---|---|
| Identifiant | InBIO Barcoding Initiative |
| Financement | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| Description du domaine d'étude / de recherche | Specimens were collected in 12 continental Portugal districts. Sixty different localities were, almost half of which (47%) from the Bragança district. Sampling was conducted from 2015 to 2019, with 39% of the specimens collected in the year 2018. |
| Description du design | Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens resulted from fieldwork collection by direct search of specimens or by sweeping the vegetation. |
Les personnes impliquées dans le projet:
- Auteur
Méthodes d'échantillonnage
The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.
| Etendue de l'étude | Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions. |
|---|---|
| Contrôle qualité | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org). |
Description des étapes de la méthode:
- Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
- Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Données de collection
| Nom de la collection | InBIO Barcoding Initiative |
|---|---|
| Identifiant de collection | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Méthode de conservation des spécimens | Alcohol |
|---|
| Unités de conservation | Entre (nombre minimal) 1 et (nombre maximal) 133 Voucher tube , Entre (nombre minimal) 1 et (nombre maximal) 135 DNA extractions |
|---|
Citations bibliographiques
- Sousa, P., Grosso-Silva, J.M., Andrade, R., Chaves, C., Pinto, J., Paupério, J., Beja, P. & Ferreira, S. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Hemiptera 01. Biodiversity Data Journal, 9: e65314. doi: 10.3897/BDJ.9.e65314
Métadonnées additionnelles
| Objet | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identifiants alternatifs | 963a25a6-839c-4bff-9889-97fc8385f564 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_hemiptera_01 |