説明
The dataset contains 135 records of Hemiptera species collected from 2015 to 2019 in continental Portugal (Sousa et al., 2021). The species represented in the dataset, 90 in total, correspond to about 7.5% of the known true bugs diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Hemiptera. Specimens were captured during fieldwork by direct search of specimens or by sweeping the vegetation and stored in 96% ethanol. All specimens were identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、135 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Sousa, P., Grosso-Silva, J. M., Andrade, R., Beja, P., Ferreira, S. (2021): The InBIO Barcoding Initiative Database: Hemiptera 01. v1.2 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/8hn25y
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 963a25a6-839c-4bff-9889-97fc8385f564が割り当てられています。 GBIF Portugal によって承認されたデータ パブリッシャーとして GBIF に登録されているCIBIO (Research Center in Biodiversity and Genetic Resources) Portugal が、このリソースをパブリッシュしました。
キーワード
Hemiptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
連絡先
- メタデータ提供者 ●
- 最初のデータ採集者
http://orcid.org/0000-0002-5859-9656
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
地理的範囲
Continental Portugal.
| 座標（緯度経度） | 南 西 [37.257, -9.465], 北 東 [41.979, -6.344] |
|---|
生物分類学的範囲
This dataset is composed of data relating to 135 Hemiptera specimens. All specimens were determined to species level or below. Overall, 90 species and one subspecies are represented in the dataset. These species belong to 31 families. The Pentatomidae family accounts for 20% of the total collected specimens, and no other family accounts for more than 8%. The Pentatomidae and Miridae families combined account for 26% of the total taxa represented, and no other family accounts for more than 7%. Eleven families are represented by a single taxon and ten by two taxa.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Hemiptera |
| Family | Acanthosomatidae, Alydidae, Aphalaridae, Aphrophoridae, Aradidae, Auchenorrhyncha, Berytidae, Cercopidae, Cicadellidae, Cicadidae, Coreidae, Corixidae, Cydnidae, Delphacidae, Dictyopharidae, Flatidae, Gerridae, Heteroptera, Hydrometridae, Lygaeidae, Membracidae, Miridae, Nabidae, Nepidae, Notonectidae, Pentatomidae, Potamocoridae, Pyrrhocoridae, Reduviidae, Rhopalidae, Rhyparochromidae, Stenocephalidae, Thaumastocoridae |
時間的範囲
| 開始日 / 終了日 | 2015-03-16 / 2019-09-20 |
|---|
プロジェクトデータ
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| タイトル | The InBIO Barcoding Initiative Database: Hemiptera 01 |
|---|---|
| 識別子 | InBIO Barcoding Initiative |
| ファンデイング | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| Study Area Description | Specimens were collected in 12 continental Portugal districts. Sixty different localities were, almost half of which (47%) from the Bragança district. Sampling was conducted from 2015 to 2019, with 39% of the specimens collected in the year 2018. |
| 研究の意図、目的、背景など（デザイン） | Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens resulted from fieldwork collection by direct search of specimens or by sweeping the vegetation. |
プロジェクトに携わる要員:
- 論文著者
収集方法
The studied material was collected in 60 different localities from continental Portugal. Sampling was conducted between 2015 and 2019 on a wide range of habitats, both by direct search of specimens or using a hand-held sweep net. Collected specimens were examined using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature.
| Study Extent | Specimens were collected in 60 different localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions. |
|---|---|
| Quality Control | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org). |
Method step description:
- Specimens were collected in 60 different localities of continental Portugal. Fieldwork was carried out between 2015 and 2019.
- Specimens were collected during fieldwork by direct search of specimens or by sweeping the vegetation and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- Specimens were morphologically identified with the assistance of a stereoscopic microscope and using the available literature. A subset (23%) was identified using the BOLD Identification Engine directly.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For one specimens of Ceraleptus lividus only a 418 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
コレクションデータ
| コレクション名 | InBIO Barcoding Initiative |
|---|---|
| コレクション識別子 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 標本保存方法 | Alcohol |
|---|
| Curatorialユニット | 範囲 1 ～ 133 Voucher tube , 範囲 1 ～ 135 DNA extractions |
|---|
書誌情報の引用
- Sousa, P., Grosso-Silva, J.M., Andrade, R., Chaves, C., Pinto, J., Paupério, J., Beja, P. & Ferreira, S. (2021). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Hemiptera 01. Biodiversity Data Journal, 9: e65314. doi: 10.3897/BDJ.9.e65314
追加のメタデータ
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 代替識別子 | 963a25a6-839c-4bff-9889-97fc8385f564 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_hemiptera_01 |