Descripción
The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 203 registros.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Ferreira S, Andrade R, Gonçalves A R, Sousa P, Beja P (2019): The InBIO Barcoding Initiative Database: Diptera 01. v1.4. CIBIO-InBIO (Research Center in Biodiversity and Genetic Resources), Portugal. Occurrence dataset. https://doi.org/10.15468/q1bvt3
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 21feb87f-62f2-4765-8aa6-6953ee5e09d6. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Portugal.
Palabras clave
Diptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contactos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
http://orcid.org/0000-0002-6884-3966
- Proveedor De Los Metadatos ●
- Originador
- Proveedor De Los Metadatos ●
- Originador
- Proveedor De Los Metadatos ●
- Originador
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
Cobertura geográfica
Continental Portugal
| Coordenadas límite | Latitud Mínima Longitud Mínima [40,054, -8,774], Latitud Máxima Longitud Máxima [42,002, -6,274] |
|---|
Cobertura taxonómica
This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Orden | Diptera |
| Familia | Anisopodidae, Anthomyiidae, Asilidae, Bibionidae, Bombyliidae, Calliphoridae, Canacidae, Chloropidae, Conopidae, Diastatidae, Dolichopodidae, Drosophilidae, Ephydridae, Fanniidae, Heleomyzidae, Hippoboscidae, Hybotidae, Lauxaniidae, Lonchaeidae, Micropezidae, Muscidae, Nemestrinidae, Opetiidae, Opomyzidae, Pallopteridae, Periscelididae, Platystomatidae, Psychodidae, Ptychopteridae, Rhiniidae, Rhinophoridae, Sarcophagidae, Scathophagidae, Sciomyzidae, Stratiomyidae, Syrphidae, Tabanidae, Tachinidae, Tephritidae, Trichoceridae, Ulidiidae |
Cobertura temporal
| Fecha Inicial / Fecha Final | 2014-05-27 / 2018-07-07 |
|---|
Datos del proyecto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: Diptera 01 |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Fuentes de Financiación | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| Descripción del área de estudio | Specimens were collected in eight continental Portugal districts. Fifty-nine different localities were sampled encompassing a great variety of habitats. Sampling was conducted from 2014 to 2018, with more than half of the specimens (62.6%) collected in the year 2017. |
| Descripción del diseño | Specimens in this dataset were collected in field work carried out between 2014 and 2018. Specimens resulted from field work directed specifically for the sampling of Diptera using different methodologies. |
Personas asociadas al proyecto:
- Autor
- Autor
Métodos de muestreo
The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)
| Área de Estudio | Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017. |
|---|---|
| Control de Calidad | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. |
Descripción de la metodología paso a paso:
- Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
- Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Datos de la colección
| Nombre de la Colección | InBIO Barcoding Initiative |
|---|---|
| Identificador de la Colección | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Métodos de preservación de los ejemplares | Alcohol |
|---|
| Unidades curatoriales | Entre 1 y 203 Voucher tube , Entre 1 y 203 DNA extractions |
|---|
Referencias bibliográficas
- Ferreira, S.A., Andrade, R., Gonçalves, A.R., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 01. Biodiversity Data Journal, 8: e49985. https://doi.org/10.3897/BDJ.8.e49985
Metadatos adicionales
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identificadores alternativos | 21feb87f-62f2-4765-8aa6-6953ee5e09d6 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_i |