說明
The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 203 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Ferreira S, Andrade R, Gonçalves A R, Sousa P, Beja P (2019): The InBIO Barcoding Initiative Database: Diptera 01. v1.4. CIBIO-InBIO (Research Center in Biodiversity and Genetic Resources), Portugal. Occurrence dataset. https://doi.org/10.15468/q1bvt3
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 21feb87f-62f2-4765-8aa6-6953ee5e09d6。 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。
關鍵字
Diptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
http://orcid.org/0000-0002-6884-3966
- 元數據提供者 ●
- 出處
- Researcher
- 元數據提供者 ●
- 出處
- Master student
- Campo Grande
- 元數據提供者 ●
- 出處
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
- 元數據提供者 ●
- 出處 ●
- 連絡人
- Coordinating Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
地理涵蓋範圍
Continental Portugal
| 界定座標範圍 | 緯度南界 經度西界 [40.054, -8.774], 緯度北界 經度東界 [42.002, -6.274] |
|---|
分類群涵蓋範圍
This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Diptera |
| Family | Anisopodidae, Anthomyiidae, Asilidae, Bibionidae, Bombyliidae, Calliphoridae, Canacidae, Chloropidae, Conopidae, Diastatidae, Dolichopodidae, Drosophilidae, Ephydridae, Fanniidae, Heleomyzidae, Hippoboscidae, Hybotidae, Lauxaniidae, Lonchaeidae, Micropezidae, Muscidae, Nemestrinidae, Opetiidae, Opomyzidae, Pallopteridae, Periscelididae, Platystomatidae, Psychodidae, Ptychopteridae, Rhiniidae, Rhinophoridae, Sarcophagidae, Scathophagidae, Sciomyzidae, Stratiomyidae, Syrphidae, Tabanidae, Tachinidae, Tephritidae, Trichoceridae, Ulidiidae |
時間涵蓋範圍
| 起始日期 / 結束日期 | 2014-05-27 / 2018-07-07 |
|---|
計畫資料
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| 計畫名稱 | The InBIO Barcoding Initiative Database: Diptera 01 |
|---|---|
| 辨識碼 | InBIO Barcoding Initiative |
| 經費來源 | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| 研究區域描述 | Specimens were collected in eight continental Portugal districts. Fifty-nine different localities were sampled encompassing a great variety of habitats. Sampling was conducted from 2014 to 2018, with more than half of the specimens (62.6%) collected in the year 2017. |
| 研究設計描述 | Specimens in this dataset were collected in field work carried out between 2014 and 2018. Specimens resulted from field work directed specifically for the sampling of Diptera using different methodologies. |
參與計畫的人員:
- 作者
- 作者
取樣方法
The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)
| 研究範圍 | Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017. |
|---|---|
| 品質控管 | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. |
方法步驟描述:
- Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
- Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
收藏資料
| 蒐藏名稱 | InBIO Barcoding Initiative |
|---|---|
| 蒐藏編號 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 標本保存方法 | Alcohol |
|---|
| 管理單位 | 在...之間 1 和 203 Voucher tube , 在...之間 1 和 203 DNA extractions |
|---|
引用文獻
- Ferreira, S.A., Andrade, R., Gonçalves, A.R., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 01. Biodiversity Data Journal, 8: e49985. https://doi.org/10.3897/BDJ.8.e49985
額外的詮釋資料
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 替代的識別碼 | 21feb87f-62f2-4765-8aa6-6953ee5e09d6 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_i |