Description
The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 203 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Ferreira S, Andrade R, Gonçalves A R, Sousa P, Beja P (2019): The InBIO Barcoding Initiative Database: Diptera 01. v1.4. CIBIO-InBIO (Research Center in Biodiversity and Genetic Resources), Portugal. Occurrence dataset. https://doi.org/10.15468/q1bvt3
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 21feb87f-62f2-4765-8aa6-6953ee5e09d6. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.
Mots-clé
Diptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contacts
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
http://orcid.org/0000-0002-6884-3966
- Fournisseur Des Métadonnées ●
- Créateur
- Researcher
- Fournisseur Des Métadonnées ●
- Créateur
- Master student
- Campo Grande
- Fournisseur Des Métadonnées ●
- Créateur
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Coordinating Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
Couverture géographique
Continental Portugal
| Enveloppe géographique | Sud Ouest [40,054, -8,774], Nord Est [42,002, -6,274] |
|---|
Couverture taxonomique
This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Diptera |
| Family | Anisopodidae, Anthomyiidae, Asilidae, Bibionidae, Bombyliidae, Calliphoridae, Canacidae, Chloropidae, Conopidae, Diastatidae, Dolichopodidae, Drosophilidae, Ephydridae, Fanniidae, Heleomyzidae, Hippoboscidae, Hybotidae, Lauxaniidae, Lonchaeidae, Micropezidae, Muscidae, Nemestrinidae, Opetiidae, Opomyzidae, Pallopteridae, Periscelididae, Platystomatidae, Psychodidae, Ptychopteridae, Rhiniidae, Rhinophoridae, Sarcophagidae, Scathophagidae, Sciomyzidae, Stratiomyidae, Syrphidae, Tabanidae, Tachinidae, Tephritidae, Trichoceridae, Ulidiidae |
Couverture temporelle
| Date de début / Date de fin | 2014-05-27 / 2018-07-07 |
|---|
Données sur le projet
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Titre | The InBIO Barcoding Initiative Database: Diptera 01 |
|---|---|
| Identifiant | InBIO Barcoding Initiative |
| Financement | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| Description du domaine d'étude / de recherche | Specimens were collected in eight continental Portugal districts. Fifty-nine different localities were sampled encompassing a great variety of habitats. Sampling was conducted from 2014 to 2018, with more than half of the specimens (62.6%) collected in the year 2017. |
| Description du design | Specimens in this dataset were collected in field work carried out between 2014 and 2018. Specimens resulted from field work directed specifically for the sampling of Diptera using different methodologies. |
Les personnes impliquées dans le projet:
- Auteur
- Auteur
Méthodes d'échantillonnage
The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)
| Etendue de l'étude | Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017. |
|---|---|
| Contrôle qualité | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. |
Description des étapes de la méthode:
- Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
- Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Données de collection
| Nom de la collection | InBIO Barcoding Initiative |
|---|---|
| Identifiant de collection | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Méthode de conservation des spécimens | Alcohol |
|---|
| Unités de conservation | Entre (nombre minimal) 1 et (nombre maximal) 203 Voucher tube , Entre (nombre minimal) 1 et (nombre maximal) 203 DNA extractions |
|---|
Citations bibliographiques
- Ferreira, S.A., Andrade, R., Gonçalves, A.R., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 01. Biodiversity Data Journal, 8: e49985. https://doi.org/10.3897/BDJ.8.e49985
Métadonnées additionnelles
| Objet | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identifiants alternatifs | 21feb87f-62f2-4765-8aa6-6953ee5e09d6 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_i |