説明
The dataset contains 203 records of Diptera species collected from 2014 to 2018 in continental Portugal (Ferreira et al., 2020). The species represented in the dataset, 154 in total, correspond to about 10% of the known fly diversity of continental Portugal, and contribute to the knowledge on the DNA barcodes and distribution of Portuguese Diptera. Specimens were captured during fieldwork directed specifically for the sampling of Diptera using different methodologies and stored in 96% ethanol. All specimens were morphologically identified to species level. A tissue sample, usually a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、203 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Ferreira S, Andrade R, Gonçalves A R, Sousa P, Beja P (2019): The InBIO Barcoding Initiative Database: Diptera 01. v1.4. CIBIO-InBIO (Research Center in Biodiversity and Genetic Resources), Portugal. Occurrence dataset. https://doi.org/10.15468/q1bvt3
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 21feb87f-62f2-4765-8aa6-6953ee5e09d6が割り当てられています。 GBIF Portugal によって承認されたデータ パブリッシャーとして GBIF に登録されているCIBIO (Research Center in Biodiversity and Genetic Resources) Portugal が、このリソースをパブリッシュしました。
キーワード
Diptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
連絡先
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
http://orcid.org/0000-0002-6884-3966
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
地理的範囲
Continental Portugal
| 座標（緯度経度） | 南 西 [40.054, -8.774], 北 東 [42.002, -6.274] |
|---|
生物分類学的範囲
This dataset is composed of data relating to 203 Diptera specimens. All specimens were determined to species level. Overall, 154 species are represented in the dataset. These species belong to 41 families. Five families account for 56% of the total collected specimens, Syrphidae, Muscidae, Tachinidae, Calliphoridae and Chloropidae. These five families account for 54% of the total species represented. Nineteen families are represented by a single species.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Diptera |
| Family | Anisopodidae, Anthomyiidae, Asilidae, Bibionidae, Bombyliidae, Calliphoridae, Canacidae, Chloropidae, Conopidae, Diastatidae, Dolichopodidae, Drosophilidae, Ephydridae, Fanniidae, Heleomyzidae, Hippoboscidae, Hybotidae, Lauxaniidae, Lonchaeidae, Micropezidae, Muscidae, Nemestrinidae, Opetiidae, Opomyzidae, Pallopteridae, Periscelididae, Platystomatidae, Psychodidae, Ptychopteridae, Rhiniidae, Rhinophoridae, Sarcophagidae, Scathophagidae, Sciomyzidae, Stratiomyidae, Syrphidae, Tabanidae, Tachinidae, Tephritidae, Trichoceridae, Ulidiidae |
時間的範囲
| 開始日 / 終了日 | 2014-05-27 / 2018-07-07 |
|---|
プロジェクトデータ
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| タイトル | The InBIO Barcoding Initiative Database: Diptera 01 |
|---|---|
| 識別子 | InBIO Barcoding Initiative |
| ファンデイング | This project is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA—Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), by EDP Biodiversity Chair, and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). P.S. was funded by the project ECOLIVES – Fostering sustainable management in Mediterranean olive farms: pest control services provided by wild species as incentives for biodiversity conservation (PTDC/AAG-REC/6480/2014/) supported by Portuguese national funds by FCT/MCTES and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE – Programa Operacional Factores de Competitividade (POFC). |
| Study Area Description | Specimens were collected in eight continental Portugal districts. Fifty-nine different localities were sampled encompassing a great variety of habitats. Sampling was conducted from 2014 to 2018, with more than half of the specimens (62.6%) collected in the year 2017. |
| 研究の意図、目的、背景など（デザイン） | Specimens in this dataset were collected in field work carried out between 2014 and 2018. Specimens resulted from field work directed specifically for the sampling of Diptera using different methodologies. |
プロジェクトに携わる要員:
- 論文著者
- 論文著者
収集方法
The studied material was collected in 59 different localities from the northern half of continental Portugal (Fig. 1, Table 1). Sampling was conducted between 2014 and 2018 on a wide range of habitats, using mainly hand-held sweep nets or direct search of specimens. Collected specimens were examined both dry and in alcohol using a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and stored in 96% ethanol for downstream molecular analysis. Morphological identification was performed based on keys and descriptions from literature (Suppl. material 1)
| Study Extent | Specimens were collected in 59 different localities of continental Portugal. Sampling was conducted from 2014 to 2018, in several field work expeditions. More than half of the specimens (62.6%) were collected in the year 2017. |
|---|---|
| Quality Control | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. |
Method step description:
- Specimens were collected in 59 different localities of continental Portugal. Fieldwork was carried out between 2014 and 2018.
- Specimens were collected during fieldwork by direct search of specimens or by the use of entomological nets to intercept specimens flight or to sweep the vegetation. Captured specimens were preserved in 96% alcohol.
- Specimens were morphologically identified with the assistance of a stereoscopic microscope (Optika ST-30-2LR, 20x-40x) and using available literature.
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. Specimens and DNA extracts were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- All obtained sequences were submitted to BOLD and GenBank databases. The morphological identification of each sequenced specimen, when available, was contrasted with the results of the BLAST of the newly generated DNA barcodes in the BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
コレクションデータ
| コレクション名 | InBIO Barcoding Initiative |
|---|---|
| コレクション識別子 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 標本保存方法 | Alcohol |
|---|
| Curatorialユニット | 範囲 1 ～ 203 Voucher tube , 範囲 1 ～ 203 DNA extractions |
|---|
書誌情報の引用
- Ferreira, S.A., Andrade, R., Gonçalves, A.R., Sousa, P., Paupério, J., Fonseca, N.A. & Beja, P. (2020). The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 01. Biodiversity Data Journal, 8: e49985. https://doi.org/10.3897/BDJ.8.e49985
追加のメタデータ
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 代替識別子 | 21feb87f-62f2-4765-8aa6-6953ee5e09d6 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_diptera_i |