The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
Última versión Publicado por CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal en Jan 5, 2021
The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros
Los datos en este registros biológicos recurso han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 5 registros.
Este IPT archiva los datos, sirviendo así como repositorio de datos. Los datos y metadatos están disponibles para descargar en la sección de descargas. La tabla de versiones muestra otras versiones del recurso que se han hecho accesibles al público y permite el seguimiento de los cambios hechos al recurso en el tiempo.
Descargas
Descargue la última versión de los datos como un Archivo Darwin Core (DwC-A) o los metadatos como EML o RTF:
| Datos como un archivo DwC-A | descargar 5 registros en Inglés (7 KB) - Frecuencia de actualización: cuando sea necesario |
|---|---|
| Metadatos como un archivo EML | descargar en Inglés (22 KB) |
| Metadatos como un archivo RTF | descargar en Inglés (17 KB) |
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Corley M, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/9z8bpm
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: cd11c12e-1f1c-4ec6-8b28-c57425956915. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso, y está registrado en GBIF como un publicador de datos avalado por GBIF Portugal.
Palabras Clave
Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contactos
¿Quién creó el recurso?:
¿Quién puede resolver dudas acerca del recurso?:
¿Quién documentó los metadatos?:
Cobertura Geográfica
Continental Portugal.
| Coordenadas límite | Latitud Mínima Longitud Mínima [41.422, -8.169], Latitud Máxima Longitud Máxima [42.003, -7.004] |
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Cobertura Taxonómica
This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Orden | Lepidoptera |
| Familia | Tortricidae |
Cobertura Temporal
| Fecha Inicial / Fecha Final | 2012-06-14 / 2016-05-31 |
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Datos del Proyecto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae) |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Fuentes de Financiación | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). |
| Descripción del Área de Estudio | Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016. |
| Descripción del Diseño | Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night. |
Personas asociadas al proyecto:
Métodos de Muestreo
Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Área de Estudio | Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016. |
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| Control de Calidad | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Descripción de la metodología paso a paso:
- Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
- The captured specimens were preserved in dry storage.
- The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Datos de la Colección
| Nombre de la Colección | InBIO Barcoding Initiative |
|---|---|
| Identificador de la Colección | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador de la Colección Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservación de los ejemplares | Alcohol |
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| Unidades Curatoriales | Entre 1 y 5 Voucher tube , Entre 1 y 5 DNA extractions |
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Referencias Bibliográficas
- Corley, M.F.V. & Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600. https://doi.org/10.11646/zootaxa.4221.5.7
- Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
- Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
- Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3. http://dx.doi.org/10.4081/jear.2013.e1
Metadatos Adicionales
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
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| Identificadores Alternativos | cd11c12e-1f1c-4ec6-8b28-c57425956915 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana |