The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)

Dernière version Publié par CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal le Jan 5, 2021 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Enregistrements de données

Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 5 enregistrements.

Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées des ressources sont disponibles au téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.

Téléchargements

Téléchargez la dernière version de la ressource en tant qu'Archive Darwin Core (DwC-A), ou les métadonnées de la ressource au format EML ou RTF :

Données sous forme de fichier DwC-A (zip) télécharger 5 enregistrements dans Anglais (7 KB) - Fréquence de mise à jour: quand cela est nécessaire
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Versions

Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.

Comment citer

Les chercheurs doivent citer cette ressource comme suit:

Corley M, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/9z8bpm

Droits

Les chercheurs doivent respecter la déclaration de droits suivante:

L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Enregistrement GBIF

Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : cd11c12e-1f1c-4ec6-8b28-c57425956915.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.

Mots-clé

Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence

Contacts

Personne ayant créé cette ressource:

Martin Corley
Researcher
Pucketty Farm Cottage SN7 8JP Faringdon Oxfordshire GB
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Personne pouvant répondre aux questions sur la ressource:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Personne ayant renseigné les métadonnées:

Martin Corley
Researcher
Pucketty Farm Cottage SN7 8JP Faringdon Oxfordshire GB
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Sousa
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Couverture géographique

Continental Portugal.

Enveloppe géographique Sud Ouest [41.422, -8.169], Nord Est [42.003, -7.004]

Couverture taxonomique

This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Lepidoptera
Family  Tortricidae

Couverture temporelle

Date de début / Date de fin 2012-06-14 / 2016-05-31

Données sur le projet

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Titre The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae)
Identifiant InBIO Barcoding Initiative
Financement InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).
Description du domaine d'étude / de recherche Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016.
Description du design Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night.

Les personnes impliquées dans le projet:

Méthodes d'échantillonnage

Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

Etendue de l'étude Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016.
Contrôle qualité The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Description des étapes de la méthode:

  1. Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
  2. The captured specimens were preserved in dry storage.
  3. The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
  4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  5. The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
  6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Données de collection

Nom de la collection InBIO Barcoding Initiative
Identifiant de collection 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Identifiant de la collection parente CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Méthode de conservation des spécimens Alcohol
Unités de conservation Entre (nombre minimal) 1 et (nombre maximal) 5 Voucher tube ,  Entre (nombre minimal) 1 et (nombre maximal) 5 DNA extractions

Citations bibliographiques

  1. Corley, M.F.V. & Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600. https://doi.org/10.11646/zootaxa.4221.5.7
  2. Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
  3. Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
  4. Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3. http://dx.doi.org/10.4081/jear.2013.e1

Métadonnées additionnelles

Objet The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Identifiants alternatifs cd11c12e-1f1c-4ec6-8b28-c57425956915
http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana