Description
The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 5 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Corley M, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/9z8bpm
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : cd11c12e-1f1c-4ec6-8b28-c57425956915. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.
Mots-clé
Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contacts
- Fournisseur Des Métadonnées ●
- Créateur
- Researcher
http://orcid.org/0000-0003-4240-8007
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
- Fournisseur Des Métadonnées
- Post-Doc Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
- Coordinating Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
- Fournisseur Des Métadonnées
- Coordinating Researcher
- Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
- +351 252660411
Couverture géographique
Continental Portugal.
| Enveloppe géographique | Sud Ouest [41,422, -8,169], Nord Est [42,003, -7,004] |
|---|
Couverture taxonomique
This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Lepidoptera |
| Family | Tortricidae |
Couverture temporelle
| Date de début / Date de fin | 2012-06-14 / 2016-05-31 |
|---|
Données sur le projet
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Titre | The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae) |
|---|---|
| Identifiant | InBIO Barcoding Initiative |
| Financement | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). |
| Description du domaine d'étude / de recherche | Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016. |
| Description du design | Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night. |
Les personnes impliquées dans le projet:
Méthodes d'échantillonnage
Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Etendue de l'étude | Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016. |
|---|---|
| Contrôle qualité | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Description des étapes de la méthode:
- Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
- The captured specimens were preserved in dry storage.
- The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Données de collection
| Nom de la collection | InBIO Barcoding Initiative |
|---|---|
| Identifiant de collection | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identifiant de la collection parente | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Méthode de conservation des spécimens | Alcohol |
|---|
| Unités de conservation | Entre (nombre minimal) 1 et (nombre maximal) 5 Voucher tube , Entre (nombre minimal) 1 et (nombre maximal) 5 DNA extractions |
|---|
Citations bibliographiques
- Corley, M.F.V. & Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600. https://doi.org/10.11646/zootaxa.4221.5.7
- Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
- Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
- Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3. http://dx.doi.org/10.4081/jear.2013.e1
Métadonnées additionnelles
| Objet | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identifiants alternatifs | cd11c12e-1f1c-4ec6-8b28-c57425956915 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana |