Descrição
The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 5 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Corley M, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/9z8bpm
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: cd11c12e-1f1c-4ec6-8b28-c57425956915. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Portugal.
Palavras-chave
Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contatos
- Provedor Dos Metadados ●
- Originador
http://orcid.org/0000-0003-4240-8007
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- Provedor Dos Metadados
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- Provedor Dos Metadados
Cobertura Geográfica
Continental Portugal.
| Coordenadas delimitadoras | Sul Oeste [41,422, -8,169], Norte Leste [42,003, -7,004] |
|---|
Cobertura Taxonômica
This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Ordem | Lepidoptera |
| Família | Tortricidae |
Cobertura Temporal
| Data Inicial / Data final | 2012-06-14 / 2016-05-31 |
|---|
Dados Sobre o Projeto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae) |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Financiamento | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). |
| Descrição da Área de Estudo | Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016. |
| Descrição do Design | Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night. |
O pessoal envolvido no projeto:
Métodos de Amostragem
Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Área de Estudo | Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016. |
|---|---|
| Controle de Qualidade | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Descrição dos passos do método:
- Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
- The captured specimens were preserved in dry storage.
- The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Dados de Coleção
| Nome da Coleção | InBIO Barcoding Initiative |
|---|---|
| Identificador da Coleção | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador da Coleção Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservação do espécime | Álcool |
|---|
| Unidades de Curadoria | Entre 1 e 5 Voucher tube , Entre 1 e 5 DNA extractions |
|---|
Citações bibliográficas
- Corley, M.F.V. & Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600. https://doi.org/10.11646/zootaxa.4221.5.7
- Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
- Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
- Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3. http://dx.doi.org/10.4081/jear.2013.e1
Metadados Adicionais
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identificadores alternativos | cd11c12e-1f1c-4ec6-8b28-c57425956915 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana |