説明
The dataset contains five records of moth specimens of the genus Isotrias collected from 2012 to 2016 in continental Portugal. The study of these specimens resulted in the discovery of sexual dimorphism in the species Isotrias penedana Trematerra, 2013, and in the description of the previously unknown female of the species in Corley and Ferreira (2017). Specimens were detected and captured by direct search of the environment during the day, with adults’ hand-netted, and by night using mercury-vapor lamps to attract the insects. Captured specimens were identified to species level and kept in dry storage. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、5 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Corley M, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/9z8bpm
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: cd11c12e-1f1c-4ec6-8b28-c57425956915が割り当てられています。 GBIF Portugal によって承認されたデータ パブリッシャーとして GBIF に登録されているCIBIO (Research Center in Biodiversity and Genetic Resources) Portugal が、このリソースをパブリッシュしました。
キーワード
Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
連絡先
- メタデータ提供者 ●
- 最初のデータ採集者
http://orcid.org/0000-0003-4240-8007
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
- メタデータ提供者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
- メタデータ提供者
地理的範囲
Continental Portugal.
| 座標（緯度経度） | 南 西 [41.422, -8.169], 北 東 [42.003, -7.004] |
|---|
生物分類学的範囲
This dataset is composed of data relating to 5 Lepidoptera specimens. All specimens were determined to species level. All specimens belong to two species of the genus Isotrias Meyrick, 1895 and the family Tortricidae.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Lepidoptera |
| Family | Tortricidae |
時間的範囲
| 開始日 / 終了日 | 2012-06-14 / 2016-05-31 |
|---|
プロジェクトデータ
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| タイトル | The InBIO Barcoding Initiative Database: DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae) |
|---|---|
| 識別子 | InBIO Barcoding Initiative |
| ファンデイング | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981, from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement No 286431 and from project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). |
| Study Area Description | Specimens were collected in continental Portugal, in two districts of the North area of the country, Viana do Castelo and Bragança, in two different localities. Sampling was conducted from 2012 to 2016. |
| 研究の意図、目的、背景など（デザイン） | Specimens in this dataset were collected in field work carried out between 2012 to 2016. Specimens were captured by direct search of the environment during the afternoon, and using mercury-vapor lamps by night. |
プロジェクトに携わる要員:
収集方法
Sampling of specimens was done at two different localities of continental Portugal. Sampling was conducted between 2012 to 2016. Captured specimens were kept in dry storage in the Research Collection of Martin Corley, while DNA extracts were stored in 96% ethanol at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Study Extent | Specimens were collected in two different localities of northerner continental Portugal. Sampling was conducted from 2012 to 2016. |
|---|---|
| Quality Control | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Method step description:
- Sampling of specimens was done at two different localities of northern continental Portugal, between 2012 and 2016. Specimens were detected and captured by direct search with an hand-netted during the afternoon, and by night using mercury-vapor lamps as lures.
- The captured specimens were preserved in dry storage.
- The specimen was morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley & Ferreira, 2017, Razowski, 2002; Trematerra, 1991, 2013).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
コレクションデータ
| コレクション名 | InBIO Barcoding Initiative |
|---|---|
| コレクション識別子 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Parent Collection Identifier | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| 標本保存方法 | Alcohol |
|---|
| Curatorialユニット | 範囲 1 ～ 5 Voucher tube , 範囲 1 ～ 5 DNA extractions |
|---|
書誌情報の引用
- Corley, M.F.V. & Ferreira, S. (2017). DNA Barcoding reveals sexual dimorphism in Isotrias penedana Trematerra, 2013 (Lepidoptera: Tortricidae, Chlidanotinae). Zootaxa, 4221(5): 594–600. https://doi.org/10.11646/zootaxa.4221.5.7
- Razowski, J. (2002) Tortricidae of Europe. 1. Tortricinae and Chlidanotinae. František Slamka, Bratislava. 247 pp.
- Trematerra, P. (1991) Nuovi dati geonemici e descrizione della femmina di Isotrias joannisana (Turati, 1921) (Lepidoptera Tortricidae). Fragmenta Entomologia, 23 (1), 53–58.
- Trematerra, P. (2013) Isotrias penedana sp. n., a new species of Lepidoptera (Tortricidae: Chlidanotinae: Polyorthini) from Portugal. Journal of Entomological and Acarological Research, 45 ( e1), 1–3. http://dx.doi.org/10.4081/jear.2013.e1
追加のメタデータ
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 代替識別子 | cd11c12e-1f1c-4ec6-8b28-c57425956915 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_lepidoptera_isotrias_penedana |