Description
The dataset contains 11 records of Stag beetle (Lucanidae) species collected from 2015 to 2019 in continental Portugal. All specimens were identified to species level, and belong to 3 species of the Lucanidae (Coleoptera). Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (a leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences available were deposited in BOLD (Barcode of Life Data System) online database. Preserved specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Data Records
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 11 records.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Ferreira S, P. da Silva L, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae). v1.2. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/7559ru
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: f1f1225f-4247-43a3-83de-7fbd2dca7e92. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.
Keywords
Coleoptera; Lucanidae; Species; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence; Specimen; Occurrence
Contacts
- Metadata Provider ●
- Originator ●
- Point Of Contact
http://orcid.org/0000-0002-6884-3966
- Metadata Provider ●
- Originator
- Metadata Provider ●
- Originator
- Metadata Provider ●
- Originator ●
- Point Of Contact
Geographic Coverage
Continental Portugal
| Bounding Coordinates | South West [37, -9], North East [41.9, -6.7] |
|---|
Taxonomic Coverage
This dataset is composed of data relating to 11 Lucanidae (Coleoptera) specimens. All specimens were determined to species level. Three of the four Stag beetle species known to occur in continental Portugal are represented in the dataset, with Dorcus parallelipipedus as the most represented species in the dataset, accounting for seven of the eleven specimens.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Coleoptera |
| Family | Lucanidae |
Temporal Coverage
| Start Date / End Date | 2015-06-17 / 2019-09-19 |
|---|
Project Data
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Title | The InBIO Barcoding Initiative Database: Portuguese Stag beetles (Coleoptera, Lucanidae) |
|---|---|
| Identifier | InBIO Barcoding Initiative |
| Funding | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Study Area Description | Specimens were collected in five Portugal districts and a total of ten sites were sampled. Sampling was conducted from 2015 to 2019. |
| Design Description | Specimens in this dataset were collected in field work carried out between 2015 and 2019. Specimens were located by direct searches carried out during field work, which enabled the sampling of both live and dead specimens. |
The personnel involved in the project:
Sampling Methods
Specimens were collected in ten localities of continental Portugal. Sampling was conducted between 2015 and 2019, by direct search. Collected specimens were identified using a binocular stereoscopic microscope and stored in 96% ethanol for downstream molecular analysis.
| Study Extent | Specimens were collected in ten localities of continental Portugal. Sampling was conducted from 2015 to 2019, in several field work expeditions. |
|---|---|
| Quality Control | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. |
Method step description:
- Sampling was conducted in continental Portugal from 2015 and 2019, and consisted in direct search. Specimens collected were stored in 96% ethanol. A tissue sample (leg) was removed from which DNA was extracted and the COI DNA barcode fragment was sequenced. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Collection Data
| Collection Name | InBIO Barcoding Initiative |
|---|---|
| Collection Identifier | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Parent Collection Identifier | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Specimen preservation methods | Alcohol |
|---|
| Curatorial Units | Between 1 and 11 Voucher tube , Between 1 and 11 DNA extractions |
|---|
Additional Metadata
| Purpose | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Alternative Identifiers | f1f1225f-4247-43a3-83de-7fbd2dca7e92 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_lucanidae |