Registros biológicos

The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)

Última versión Publicado por CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal en 3 de agosto de 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

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Descripción

The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

Registros

Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 13 registros.

Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.

Versiones

La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.

¿Cómo referenciar?

Los usuarios deben citar este trabajo de la siguiente manera:

Oliveira D, Ferreira S, P. da Silva L, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae). v1.7. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/rmj9sz

Derechos

Los usuarios deben respetar los siguientes derechos de uso:

El publicador y propietario de los derechos de este trabajo es CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).

Registro GBIF

Este recurso ha sido registrado en GBIF con el siguiente UUID: 7b84fe1d-42d0-439a-b60f-0b621f7ba93d.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Portugal.

Palabras clave

Mecoptera; Panorpidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen

Contactos

Daniel Oliveira
  • Proveedor De Los Metadatos
  • Originador
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Sónia Ferreira
  • Proveedor De Los Metadatos
  • Originador
  • Punto De Contacto
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Luís P. da Silva
  • Proveedor De Los Metadatos
  • Originador
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Brito Lopes
  • Proveedor De Los Metadatos
  • Originador
Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Pedro Sousa
  • Proveedor De Los Metadatos
  • Originador
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
  • Proveedor De Los Metadatos
  • Originador
  • Punto De Contacto
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Cobertura geográfica

Continental Portugal

Coordenadas límite Latitud Mínima Longitud Mínima [39,8, -8,7], Latitud Máxima Longitud Máxima [42, -6,4]

Cobertura taxonómica

This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal.

Reino Animalia
Filo Arthropoda
Class Insecta
Orden Mecoptera
Familia Panorpidae

Cobertura temporal

Fecha Inicial / Fecha Final 2008-06-02 / 2019-06-28

Datos del proyecto

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Título The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)
Identificador InBIO Barcoding Initiative
Fuentes de Financiación InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250.
Descripción del área de estudio Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts.
Descripción del diseño The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search.

Personas asociadas al proyecto:

Métodos de muestreo

Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.

Área de Estudio Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions.
Control de Calidad To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Descripción de la metodología paso a paso:

  1. Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
  2. The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013).
  3. DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  4. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  5. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Datos de la colección

Nombre de la Colección InBIO Barcoding Initiative
Identificador de la Colección 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Identificador de la Colección Parental CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Métodos de preservación de los ejemplares Alcohol
Unidades curatoriales Entre 1 y 13 Voucher tube ,  Entre 1 y 13 DNA extractions

Referencias bibliográficas

  1. Monserrat, V. J. and Tillier, P. (2013) ‘Sinopsis de los Mecópteros de la Península Ibérica (Insecta, Mecoptera)’, Boletín de la Sociedad Entomológica Aragonesa (S.E.A.), 53(1926), pp. 143–152.
  2. Ward, P. H. (1983) ‘Scorpion-flies of the panorpa cognata-complex in the western palaearctic region (Mecoptera)’, Journal of Natural History, 17(4), pp. 627–645. doi: 10.1080/00222938300770521. doi: 10.1080/00222938300770521

Metadatos adicionales

Propósito The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Identificadores alternativos 7b84fe1d-42d0-439a-b60f-0b621f7ba93d
http://ipt.gbif.pt/ipt/resource?r=ibi_mecoptera