Описание
The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
Записи данных
Данные этого occurrence ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 13 записей.
Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.
Версии
В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.
Как оформить ссылку
Исследователи должны дать ссылку на эту работу следующим образом:
Oliveira D, Ferreira S, P. da Silva L, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae). v1.7. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/rmj9sz
Права
Исследователи должны соблюдать следующие права:
Публикующей организацией и владельцем прав на данную работу является CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).
Регистрация в GBIF
Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 7b84fe1d-42d0-439a-b60f-0b621f7ba93d. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Portugal.
Ключевые слова
Mecoptera; Panorpidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
Контакты
- Metadata Provider ●
- Originator
http://orcid.org/0000-0002-8733-4104
- Metadata Provider ●
- Originator ●
- Point Of Contact
- Metadata Provider ●
- Originator
- Metadata Provider ●
- Originator
- Metadata Provider ●
- Originator
- Metadata Provider ●
- Originator ●
- Point Of Contact
Географический охват
Continental Portugal
| Ограничивающие координаты | Юг Запад [39,8, -8,7], Север Восток [42, -6,4] |
|---|
Таксономический охват
This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Mecoptera |
| Family | Panorpidae |
Временной охват
| Дата начала / Дата окончания | 2008-06-02 / 2019-06-28 |
|---|
Данные проекта
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Название | The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae) |
|---|---|
| Идентификатор | InBIO Barcoding Initiative |
| Финансирование | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250. |
| Описание района исследования | Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts. |
| Описание плана выполнения исследований | The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search. |
Исполнители проекта:
Методы сбора
Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.
| Охват исследования | Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions. |
|---|---|
| Контроль качества | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). |
Описание этапа методики:
- Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013).
- DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Данные коллекции
| Название коллекции | InBIO Barcoding Initiative |
|---|---|
| Идентификатор коллекции | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Идентификатор родительской коллекции | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Методы сохранения образцов | Alcohol |
|---|
| Единицы хранения | Между 1 и 13 Voucher tube , Между 1 и 13 DNA extractions |
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Библиографические ссылки
- Monserrat, V. J. and Tillier, P. (2013) ‘Sinopsis de los Mecópteros de la Península Ibérica (Insecta, Mecoptera)’, Boletín de la Sociedad Entomológica Aragonesa (S.E.A.), 53(1926), pp. 143–152.
- Ward, P. H. (1983) ‘Scorpion-flies of the panorpa cognata-complex in the western palaearctic region (Mecoptera)’, Journal of Natural History, 17(4), pp. 627–645. doi: 10.1080/00222938300770521. doi: 10.1080/00222938300770521
Дополнительные метаданные
| Цель | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Альтернативные идентификаторы | 7b84fe1d-42d0-439a-b60f-0b621f7ba93d |
| http://ipt.gbif.pt/ipt/resource?r=ibi_mecoptera |