The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 13 筆紀錄。
下載最新版本的 Darwin Core Archive (DwC-A) 資源，或資源詮釋資料的 EML 或 RTF 文字檔。
Oliveira D, Ferreira S, P. da Silva L, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae). v1.7. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/rmj9sz
此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
此資源已向GBIF註冊，並指定以下之GBIF UUID: 7b84fe1d-42d0-439a-b60f-0b621f7ba93d。 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。
Mecoptera; Panorpidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
|界定座標範圍||緯度南界 經度西界 [39.8, -8.7], 緯度北界 經度東界 [42, -6.4]|
This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal.
|起始日期 / 結束日期||2008-06-02 / 2019-06-28|
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
|計畫名稱||The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)|
|辨識碼||InBIO Barcoding Initiative|
|經費來源||InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250.|
|研究區域描述||Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts.|
|研究設計描述||The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search.|
Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.
|研究範圍||Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions.|
|品質控管||To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).|
- Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013).
- DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
|蒐藏名稱||InBIO Barcoding Initiative|
|上層採集品識別碼||CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal|
|管理單位||在...之間 1 和 13 Voucher tube , 在...之間 1 和 13 DNA extractions|
- Monserrat, V. J. and Tillier, P. (2013) ‘Sinopsis de los Mecópteros de la Península Ibérica (Insecta, Mecoptera)’, Boletín de la Sociedad Entomológica Aragonesa (S.E.A.), 53(1926), pp. 143–152.
- Ward, P. H. (1983) ‘Scorpion-flies of the panorpa cognata-complex in the western palaearctic region (Mecoptera)’, Journal of Natural History, 17(4), pp. 627–645. doi: 10.1080/00222938300770521. doi: 10.1080/00222938300770521
|目的||The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.|