出現紀錄

The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)

最新版本 由 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈於 2020年8月3日 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conduc... 增加顯示內容

說明

The dataset contains 13 records of Scorpionflies (Panorpidae) species collected from 2008 to 2019 in continental Portugal. All captured specimens were identified as Panorpa meridionalis, so far the only species of Mecoptera known to exist in Portugal Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

資料紀錄

此資源出現紀錄的資料已發佈為達爾文核心集檔案(DwC-A),其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 13 筆紀錄。

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本,以便利追蹤其隨時間的變更。

下載

下載最新版本的 Darwin Core Archive (DwC-A) 資源,或資源詮釋資料的 EML 或 RTF 文字檔。

DwC-A資料集 下載 13 紀錄 在 English 中 (8 kB) - 更新頻率: 需要時
元數據EML檔 下載 在 English 中 (26 kB)
元數據RTF文字檔 下載 在 English 中 (16 kB)

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Oliveira D, Ferreira S, P. da Silva L, Lopes P B, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae). v1.7. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/rmj9sz

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 7b84fe1d-42d0-439a-b60f-0b621f7ba93d。  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源,並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。

關鍵字

Mecoptera; Panorpidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen

聯絡資訊

資源建立者:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Luís P. da Silva
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Brito Lopes
Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

可回覆此資源相關問題者:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

元數據填寫者:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Luís P. da Silva
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Brito Lopes
Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

地理涵蓋範圍

Continental Portugal

界定座標範圍 緯度南界 經度西界 [39.8, -8.7], 緯度北界 經度東界 [42, -6.4]

分類群涵蓋範圍

This dataset is composed of data relating to 13 specimens of Panorpa meridionalis, the only species of Mecoptera known to occur in Portugal.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Mecoptera
Family  Panorpidae

時間涵蓋範圍

起始日期 / 結束日期 2008-06-02 / 2019-06-28

計畫資料

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

計畫名稱 The InBIO Barcoding Initiative Database: Portuguese Scorpionflies (Mecoptera, Panorpidae)
辨識碼 InBIO Barcoding Initiative
經費來源 InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair and the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250.
研究區域描述 Specimens were captured in several field incursions from 2008 to 2019 in in ten localities in the northern half of Portugal, encompassing seven districts.
研究設計描述 The specimens in this dataset were collected in field work incursion carried out between 2008 and 2019, encompassing several sites throughout northern Portugal. Specimens of Mecoptera were detected by direct search.

參與計畫的人員:

取樣方法

Specimens were captured from 2008 to 2019 in continental Portugal by direct search of ten sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.

研究範圍 Specimens were collected in ten sites distributed among seven districts in the north of Portugal. Sampling was conducted from 2008 to 2019, in several field work expeditions.
品質控管 To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

方法步驟描述:

  1. Specimens of Mecoptera were captured in continental Portugal from 2008 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
  2. The specimens were morphologically identified resorting to available literature (Ward, 1983; Monserrat and Tillier, 2013).
  3. DNA barcodes were sequenced from 11 specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  4. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  5. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

收藏資料

蒐藏名稱 InBIO Barcoding Initiative
蒐藏編號 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
上層採集品識別碼 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
標本保存方法 Alcohol
管理單位 在...之間 1 和 13 Voucher tube ,  在...之間 1 和 13 DNA extractions

引用文獻

  1. Monserrat, V. J. and Tillier, P. (2013) ‘Sinopsis de los Mecópteros de la Península Ibérica (Insecta, Mecoptera)’, Boletín de la Sociedad Entomológica Aragonesa (S.E.A.), 53(1926), pp. 143–152.
  2. Ward, P. H. (1983) ‘Scorpion-flies of the panorpa cognata-complex in the western palaearctic region (Mecoptera)’, Journal of Natural History, 17(4), pp. 627–645. doi: 10.1080/00222938300770521. doi: 10.1080/00222938300770521

額外的詮釋資料

目的 The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
替代的識別碼 7b84fe1d-42d0-439a-b60f-0b621f7ba93d
http://ipt.gbif.pt/ipt/resource?r=ibi_mecoptera