Descripción
The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 5 registros.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Ferreira S, Paupério J, Grosso-Silva J M, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/x7yk7m
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 22f83f75-3b92-4db6-a86e-8dac512c4e65. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Portugal.
Palabras clave
Megaloptera; Sialidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contactos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
http://orcid.org/0000-0002-6884-3966
- Proveedor De Los Metadatos ●
- Originador
- Proveedor De Los Metadatos ●
- Originador
- Proveedor De Los Metadatos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
- Proveedor De Los Metadatos
Cobertura geográfica
Continental Portugal.
| Coordenadas límite | Latitud Mínima Longitud Mínima [41,527, -7,011], Latitud Máxima Longitud Máxima [41,775, -6,556] |
|---|
Cobertura taxonómica
This dataset is composed of data relating to five Megaloptera specimens. All specimens were determined to species level. All specimens belong to the genus Sialis Latreille, 1803, and the family Sialidae.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Orden | Megaloptera |
| Familia | Sialidae |
Cobertura temporal
| Fecha Inicial / Fecha Final | 2011-06-27 / 2015-04-30 |
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Datos del proyecto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Fuentes de Financiación | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Descripción del área de estudio | Specimens were collected in continental Portugal, in four different localities. Sampling was conducted in 2011 and 2015. |
| Descripción del diseño | Specimens in this dataset were collected in field work carried in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. |
Personas asociadas al proyecto:
Métodos de muestreo
Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Área de Estudio | Specimens were collected in four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. |
|---|---|
| Control de Calidad | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Descripción de la metodología paso a paso:
- Sampling of specimens was done at four different localities of continental Portugal, in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.
- The captured specimens were preserved in 96% ethanol.
- The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Monserrat, 2014).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Datos de la colección
| Nombre de la Colección | InBIO Barcoding Initiative |
|---|---|
| Identificador de la Colección | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador de la Colección Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservación de los ejemplares | Alcohol |
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| Unidades curatoriales | Entre 1 y 5 Voucher tube , Entre 1 y 5 DNA extractions |
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Referencias bibliográficas
- Ferreira, S., Paupério, J., Grosso-Silva, J.M. & Beja, P. (2019). DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. Aquatic Insects, 40(2): 173-184. https://doi.org/10.1080/01650424.2019.1571612
- Monserrat, V.J. (2014). Los megalopteros de la Península Ibérica (Insecta, Neuropterida, Megaloptera, Sialidae), Graellsia, 70(2), e009.
Metadatos adicionales
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identificadores alternativos | 22f83f75-3b92-4db6-a86e-8dac512c4e65 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_megaloptera_sialis |