Descrição
The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 5 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Ferreira S, Paupério J, Grosso-Silva J M, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/x7yk7m
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 22f83f75-3b92-4db6-a86e-8dac512c4e65. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Portugal.
Palavras-chave
Megaloptera; Sialidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
http://orcid.org/0000-0002-6884-3966
- Provedor Dos Metadados ●
- Originador
- Provedor Dos Metadados ●
- Originador
- Provedor Dos Metadados
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
- Provedor Dos Metadados
Cobertura Geográfica
Continental Portugal.
| Coordenadas delimitadoras | Sul Oeste [41,527, -7,011], Norte Leste [41,775, -6,556] |
|---|
Cobertura Taxonômica
This dataset is composed of data relating to five Megaloptera specimens. All specimens were determined to species level. All specimens belong to the genus Sialis Latreille, 1803, and the family Sialidae.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Ordem | Megaloptera |
| Família | Sialidae |
Cobertura Temporal
| Data Inicial / Data final | 2011-06-27 / 2015-04-30 |
|---|
Dados Sobre o Projeto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Financiamento | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Descrição da Área de Estudo | Specimens were collected in continental Portugal, in four different localities. Sampling was conducted in 2011 and 2015. |
| Descrição do Design | Specimens in this dataset were collected in field work carried in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. |
O pessoal envolvido no projeto:
Métodos de Amostragem
Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| Área de Estudo | Specimens were collected in four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. |
|---|---|
| Controle de Qualidade | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
Descrição dos passos do método:
- Sampling of specimens was done at four different localities of continental Portugal, in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.
- The captured specimens were preserved in 96% ethanol.
- The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Monserrat, 2014).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Dados de Coleção
| Nome da Coleção | InBIO Barcoding Initiative |
|---|---|
| Identificador da Coleção | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador da Coleção Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservação do espécime | Álcool |
|---|
| Unidades de Curadoria | Entre 1 e 5 Voucher tube , Entre 1 e 5 DNA extractions |
|---|
Citações bibliográficas
- Ferreira, S., Paupério, J., Grosso-Silva, J.M. & Beja, P. (2019). DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. Aquatic Insects, 40(2): 173-184. https://doi.org/10.1080/01650424.2019.1571612
- Monserrat, V.J. (2014). Los megalopteros de la Península Ibérica (Insecta, Neuropterida, Megaloptera, Sialidae), Graellsia, 70(2), e009.
Metadados Adicionais
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identificadores alternativos | 22f83f75-3b92-4db6-a86e-8dac512c4e65 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_megaloptera_sialis |