Occurrence

The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification

Последняя версия опубликована CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 5 января 2021 г. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of t... Больше
Домой:
Ссылка
Дата публикации:
5 января 2021 г.
Техническое обеспечение:
CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Лицензия:
CC-BY 4.0

Описание

The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Записи данных

Данные этого occurrence ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 5 записей.

Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.

Загрузки

Скачайте последнюю версию данных этого ресурса в формате Darwin Core Archive (DwC-A) или метаданных ресурса в форматах EML или RTF:

Данные в формате DwC-A Скачать 5 Записи в English (7 kB) - Частота обновления: as needed
Метаданные в формате EML Скачать в English (24 kB)
Метаданные в формате RTF Скачать в English (17 kB)

Версии

В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.

Как оформить ссылку

Исследователи должны дать ссылку на эту работу следующим образом:

Ferreira S, Paupério J, Grosso-Silva J M, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/x7yk7m

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 22f83f75-3b92-4db6-a86e-8dac512c4e65.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Portugal.

Ключевые слова

Megaloptera; Sialidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence

Контакты

Кто является создателем ресурса:

Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Joana Paupério
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
José Manuel Grosso-Silva
Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Praça Gomes Teixeira
4099-002 Porto
Porto
PT
Pedro Sousa
Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Кто может ответить на вопросы о ресурсе:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Кем заполнены метаданные:

Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Joana Paupério
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
José Manuel Grosso-Silva
Entomology Curator
Museu de História Natural e da Ciência da Universidade do Porto
Praça Gomes Teixeira
4099-002 Porto
Porto
PT
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
+351 252660411

Географический охват

Continental Portugal.

Ограничивающие координаты Юг Запад [41,527, -7,011], Север Восток [41,775, -6,556]

Таксономический охват

This dataset is composed of data relating to five Megaloptera specimens. All specimens were determined to species level. All specimens belong to the genus Sialis Latreille, 1803, and the family Sialidae.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Megaloptera
Family  Sialidae

Временной охват

Дата начала / Дата окончания 2011-06-27 / 2015-04-30

Данные проекта

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Название The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification
Идентификатор InBIO Barcoding Initiative
Финансирование InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Описание района исследования Specimens were collected in continental Portugal, in four different localities. Sampling was conducted in 2011 and 2015.
Описание плана выполнения исследований Specimens in this dataset were collected in field work carried in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.

Исполнители проекта:

Author
Sónia Ferreira
Author
Joana Paupério
Author
José Manuel Grosso-Silva
Originator
Pedro Sousa

Методы сбора

Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.

Охват исследования Specimens were collected in four different localities of continental Portugal. Sampling was conducted in 2011 and 2015.
Контроль качества The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Описание этапа методики:

  1. Sampling of specimens was done at four different localities of continental Portugal, in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.
  2. The captured specimens were preserved in 96% ethanol.
  3. The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Monserrat, 2014).
  4. DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  5. The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
  6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Данные коллекции

Название коллекции InBIO Barcoding Initiative
Идентификатор коллекции 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Идентификатор родительской коллекции CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Методы сохранения образцов Alcohol
Единицы хранения Между 1 и 5 Voucher tube ,  Между 1 и 5 DNA extractions

Библиографические ссылки

  1. Ferreira, S., Paupério, J., Grosso-Silva, J.M. & Beja, P. (2019). DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. Aquatic Insects, 40(2): 173-184. https://doi.org/10.1080/01650424.2019.1571612
  2. Monserrat, V.J. (2014). Los megalopteros de la Península Ibérica (Insecta, Neuropterida, Megaloptera, Sialidae), Graellsia, 70(2), e009.

Дополнительные метаданные

Цель The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Альтернативные идентификаторы 22f83f75-3b92-4db6-a86e-8dac512c4e65
http://ipt.gbif.pt/ipt/resource?r=ibi_megaloptera_sialis