說明
The dataset contains five records of the alderfly genus Sialis Latreille, 1803 (Megaloptera, Sialidae) collected in 2011 and 2015 in northern continental Portugal. The study of these specimens found two species previously unknown in the country, S. lutaria Linnaeus, 1758 and S. nigripes Pictet, 1865 and confirmed the presence of S. fuliginosa Pictet, 1836 in Portugal (Ferreira et al. 2019). In that work the three species were identified morphologically and confirmed with DNA barcodes. Specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. Captured specimens were identified to species level and preserved in 96% ethanol. A tissue sample, a leg, was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) and GenBank online databases. DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 5 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Ferreira S, Paupério J, Grosso-Silva J M, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/x7yk7m
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 22f83f75-3b92-4db6-a86e-8dac512c4e65。 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。
關鍵字
Megaloptera; Sialidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
聯絡資訊
- 元數據提供者 ●
- 出處 ●
- 連絡人
http://orcid.org/0000-0002-6884-3966
- 元數據提供者 ●
- 出處
- 元數據提供者 ●
- 出處
- 元數據提供者
- 元數據提供者 ●
- 出處 ●
- 連絡人
- 元數據提供者
地理涵蓋範圍
Continental Portugal.
| 界定座標範圍 | 緯度南界 經度西界 [41.527, -7.011], 緯度北界 經度東界 [41.775, -6.556] |
|---|
分類群涵蓋範圍
This dataset is composed of data relating to five Megaloptera specimens. All specimens were determined to species level. All specimens belong to the genus Sialis Latreille, 1803, and the family Sialidae.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Megaloptera |
| Family | Sialidae |
時間涵蓋範圍
| 起始日期 / 結束日期 | 2011-06-27 / 2015-04-30 |
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計畫資料
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| 計畫名稱 | The InBIO Barcoding Initiative Database: DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification |
|---|---|
| 辨識碼 | InBIO Barcoding Initiative |
| 經費來源 | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and is part of research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| 研究區域描述 | Specimens were collected in continental Portugal, in four different localities. Sampling was conducted in 2011 and 2015. |
| 研究設計描述 | Specimens in this dataset were collected in field work carried in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net. |
參與計畫的人員:
取樣方法
Sampling of specimens was done at four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. Captured specimens were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. The collected specimens were morphologically identified.
| 研究範圍 | Specimens were collected in four different localities of continental Portugal. Sampling was conducted in 2011 and 2015. |
|---|---|
| 品質控管 | The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/). |
方法步驟描述:
- Sampling of specimens was done at four different localities of continental Portugal, in 2011 and 2015. Adult specimens were detected by direct search on vegetation and rocks around river streams and captured with a hand-net.
- The captured specimens were preserved in 96% ethanol.
- The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Monserrat, 2014).
- DNA barcodes were sequenced from all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- The obtained sequences were submitted to BOLD and GenBank databases and the specimens’ morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
收藏資料
| 蒐藏名稱 | InBIO Barcoding Initiative |
|---|---|
| 蒐藏編號 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 上層採集品識別碼 | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| 標本保存方法 | Alcohol |
|---|
| 管理單位 | 在...之間 1 和 5 Voucher tube , 在...之間 1 和 5 DNA extractions |
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引用文獻
- Ferreira, S., Paupério, J., Grosso-Silva, J.M. & Beja, P. (2019). DNA barcoding of Sialis sp. (Megaloptera) in Portugal: the missing tool to species identification. Aquatic Insects, 40(2): 173-184. https://doi.org/10.1080/01650424.2019.1571612
- Monserrat, V.J. (2014). Los megalopteros de la Península Ibérica (Insecta, Neuropterida, Megaloptera, Sialidae), Graellsia, 70(2), e009.
額外的詮釋資料
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 替代的識別碼 | 22f83f75-3b92-4db6-a86e-8dac512c4e65 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_megaloptera_sialis |