The dataset contains two records of moth specimens (Lepidoptera, Depressariidae) collected in 2011 and 2015 in continental Portugal. The study of these specimens resulted in the description of a new Portuguese endemic species, Depressaria infernella Corley & Buchner, 2019 based on differences found in the male and female genitalia and DNA barcodes in Corley et al. (2019). Specimens were detected and captured using a mercury-vapor lamp to attract the insects. Captured specimens were identified to species level and preserved in 96% ethanol or in dry storage. From each specimen a tissue sample, one leg, was removed to be used for DNA extraction. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA barcode sequences were deposited in BOLD (Barcode of Life Data System) online database. Collected specimens and DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 2 records.
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Researchers should cite this work as follows:
Corley M, Ferreira S, Buchner P, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group. v1.0. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Dataset/Occurrence. https://doi.org/10.15468/gcg7ny
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The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
This resource has been registered with GBIF, and assigned the following GBIF UUID: f90de717-7b3f-483f-aca2-46c300fe36a8. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.
Lepidoptera; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Occurrence
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|Bounding Coordinates||South West [40.326, -7.573], North East [41.766, -6.902]|
This dataset is composed of data relating to two Lepidoptera specimens. Both specimens were determined to species level. The dataset is composed of two species, Depressaria infernella Corley & Buchner, 2019 and Agonopterix scopariella (Heinemann, 1870). Both species belong to the family Depressariidae.
|Start Date / End Date||2011-08-05 / 2015-06-19|
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
|Title||The InBIO Barcoding Initiative Database: Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group|
|Identifier||InBIO Barcoding Initiative|
|Funding||InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).|
|Study Area Description||The two specimens were collected in the districts of Bragança and Guarda, in Continental Portugal, in 2011 and 2015.|
|Design Description||The specimens in this dataset were collected in Continental Portugal in two field work incursions carried out in 2011 and 2015. Specimens were detected using a mercury-vapor lamps as lures.|
The personnel involved in the project:
Specimens were captured in 2011 and 2015 in continental Portugal uisng mercury-vapor lamps as lures. Captured insects were preserved in 96% ethanol or in dry storage and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected specimens were morphologically identified using a binocular stereoscopic microscope.
|Study Extent||Specimens were collected in Continental Portugal in two field work incursions carried out in 2011 and 2015.|
|Quality Control||The DNA barcoded specimen morphological identification was contrasted with the results of the Blast of the newly generated DNA barcode in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).|
Method step description:
- Specimens were captured in two localities of continental Portugal, in 2011 and 2015, using mercury-vapor lamps as lures.
- Captured specimens were preserved in 96% ethanol or in dry storage.
- The specimens were morphologically identified using a binocular stereoscopic microscope and resorting to available literature (Corley, Buchner & Ferreira, 2019; Hannemann, 1953; Palm, 1989.)
- A DNA barcode was sequenced from a tissue sample, one leg, that was removed. DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced.
- The obtained sequences were submitted to BOLD database and the specimen morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcode in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
|Collection Name||InBIO Barcoding Initiative|
|Parent Collection Identifier||CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal|
|Specimen preservation methods||Alcohol|
|Curatorial Units||Between 1 and 2 Voucher tube , Between 1 and 2 DNA extractions|
- Corley, M. F. V., Buchner, P. & Ferreira, S. (2019). Depressaria infernella Corley & Buchner, a new Iberian species of the Depressaria douglasella group (Lepidoptera: Depressariidae). SHILAP, Revista de Lepidopterología, 47(186): 293-300.
- Hannemann, H. J., 1953. Natürliche Gruppierung der europäischen Arten der Gattung Depressaria s. l. (Lep. Oecoph.).– Mitteilungen aus dem Zoologischen Museum in Berlin, 29: 269-373.
- Palm, E., 1989. Nordeuropas Prydvinger (Lepidoptera: Oecophoridae).– Danmarks Dyreliv, 4: 1-247.
|Purpose||The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.|