Description
The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Enregistrements de données
Les données de cette ressource occurrence ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 38 enregistrements.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Comment citer
Les chercheurs doivent citer cette ressource comme suit:
Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/m7f8sg
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 64704ea2-6caf-40fd-a323-641b536f5152. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Portugal.
Mots-clé
Blattodea; Cockroach; Termite; Species; Cytochrome c oxidase I (COI); Occurrence; DNA barcode; Continental Portugal
Contacts
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
http://orcid.org/0000-0002-6884-3966
- Fournisseur Des Métadonnées ●
- Créateur
- Fournisseur Des Métadonnées ●
- Créateur
- Fournisseur Des Métadonnées ●
- Créateur ●
- Personne De Contact
Couverture géographique
Continental Portugal.
| Enveloppe géographique | Sud Ouest [37,176, -8,917], Nord Est [41,892, -6,561] |
|---|
Couverture taxonomique
This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Blattodea |
| Family | Blattidae |
Couverture temporelle
| Date de début / Date de fin | 2005-08-15 / 2019-05-28 |
|---|
Données sur le projet
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Titre | The InBIO Barcoding Initiative Database: Blattodea 01 |
|---|---|
| Identifiant | InBIO Barcoding Initiative |
| Financement | InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Description du domaine d'étude / de recherche | Continental Portugal |
| Description du design | Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%). |
Les personnes impliquées dans le projet:
- Auteur
Méthodes d'échantillonnage
The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.
| Etendue de l'étude | Continental Portugal. |
|---|---|
| Contrôle qualité | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org). |
Description des étapes de la méthode:
- Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
- Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
- DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Données de collection
| Nom de la collection | InBIO Barcoding Initiative |
|---|---|
| Identifiant de collection | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identifiant de la collection parente | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Méthode de conservation des spécimens | Alcohol |
|---|
| Unités de conservation | Entre (nombre minimal) 1 et (nombre maximal) 38 Voucher tube , Entre (nombre minimal) 1 et (nombre maximal) 28 DNA extractions |
|---|
Métadonnées additionnelles
| Objet | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identifiants alternatifs | 64704ea2-6caf-40fd-a323-641b536f5152 |
| http://ipt.gbif.pt/ipt/resource?r=blattodea_1 |