説明
The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
データ レコード
この オカレンス（観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、38 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/m7f8sg
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 64704ea2-6caf-40fd-a323-641b536f5152が割り当てられています。 GBIF Portugal によって承認されたデータ パブリッシャーとして GBIF に登録されているCIBIO (Research Center in Biodiversity and Genetic Resources) Portugal が、このリソースをパブリッシュしました。
キーワード
Blattodea; Cockroach; Termite; Species; Cytochrome c oxidase I (COI); Occurrence; DNA barcode; Continental Portugal
連絡先
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
http://orcid.org/0000-0002-6884-3966
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
地理的範囲
Continental Portugal.
| 座標（緯度経度） | 南 西 [37.176, -8.917], 北 東 [41.892, -6.561] |
|---|
生物分類学的範囲
This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Blattodea |
| Family | Blattidae |
時間的範囲
| 開始日 / 終了日 | 2005-08-15 / 2019-05-28 |
|---|
プロジェクトデータ
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| タイトル | The InBIO Barcoding Initiative Database: Blattodea 01 |
|---|---|
| 識別子 | InBIO Barcoding Initiative |
| ファンデイング | InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Study Area Description | Continental Portugal |
| 研究の意図、目的、背景など（デザイン） | Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%). |
プロジェクトに携わる要員:
- 論文著者
収集方法
The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.
| Study Extent | Continental Portugal. |
|---|---|
| Quality Control | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org). |
Method step description:
- Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
- Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
- DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
コレクションデータ
| コレクション名 | InBIO Barcoding Initiative |
|---|---|
| コレクション識別子 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Parent Collection Identifier | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| 標本保存方法 | Alcohol |
|---|
| Curatorialユニット | 範囲 1 ～ 38 Voucher tube , 範囲 1 ～ 28 DNA extractions |
|---|
追加のメタデータ
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 代替識別子 | 64704ea2-6caf-40fd-a323-641b536f5152 |
| http://ipt.gbif.pt/ipt/resource?r=blattodea_1 |