Descrição
The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 38 registros.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/m7f8sg
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 64704ea2-6caf-40fd-a323-641b536f5152. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Portugal.
Palavras-chave
Blattodea; Cockroach; Termite; Species; Cytochrome c oxidase I (COI); Occurrence; DNA barcode; Continental Portugal
Contatos
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
http://orcid.org/0000-0002-6884-3966
- Provedor Dos Metadados ●
- Originador
- Provedor Dos Metadados ●
- Originador
- Provedor Dos Metadados ●
- Originador ●
- Ponto De Contato
Cobertura Geográfica
Continental Portugal.
| Coordenadas delimitadoras | Sul Oeste [37,176, -8,917], Norte Leste [41,892, -6,561] |
|---|
Cobertura Taxonômica
This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Ordem | Blattodea |
| Família | Blattidae |
Cobertura Temporal
| Data Inicial / Data final | 2005-08-15 / 2019-05-28 |
|---|
Dados Sobre o Projeto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: Blattodea 01 |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Financiamento | InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Descrição da Área de Estudo | Continental Portugal |
| Descrição do Design | Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%). |
O pessoal envolvido no projeto:
- Autor
Métodos de Amostragem
The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.
| Área de Estudo | Continental Portugal. |
|---|---|
| Controle de Qualidade | All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org). |
Descrição dos passos do método:
- Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
- Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
- Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
- DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).
Dados de Coleção
| Nome da Coleção | InBIO Barcoding Initiative |
|---|---|
| Identificador da Coleção | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador da Coleção Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservação do espécime | Álcool |
|---|
| Unidades de Curadoria | Entre 1 e 38 Voucher tube , Entre 1 e 28 DNA extractions |
|---|
Metadados Adicionais
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| Identificadores alternativos | 64704ea2-6caf-40fd-a323-641b536f5152 |
| http://ipt.gbif.pt/ipt/resource?r=blattodea_1 |