The InBIO Barcoding Initiative Database: Blattodea 01

Latest version published by CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal on Dec 14, 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 38 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Downloads

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 38 records in English (10 KB) - Update frequency: as needed
Metadata as an EML file download in English (21 KB)
Metadata as an RTF file download in English (15 KB)

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/m7f8sg

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 64704ea2-6caf-40fd-a323-641b536f5152.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.

Keywords

Blattodea; Cockroach; Termite; Species; Cytochrome c oxidase I (COI); Occurrence; DNA barcode; Continental Portugal

Contacts

Who created the resource:

Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Horst Bohn
Professor
Zoologische Staatssammlung München Staatliche Naturwissenschaftliche Sammlungen Bayerns (SNSB), Zoologische Staatssammlung München, Münchhausenstraße 21 81247 München Munich Bavaria DE +49 89 8107144
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Who can answer questions about the resource:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Who filled in the metadata:

Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Horst Bohn
Professor
Zoologische Staatssammlung München Staatliche Naturwissenschaftliche Sammlungen Bayerns (SNSB), Zoologische Staatssammlung München, Münchhausenstraße 21 81247 München Munich Bavaria DE +49 89 8107144
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Geographic Coverage

Continental Portugal.

Bounding Coordinates South West [37.176, -8.917], North East [41.892, -6.561]

Taxonomic Coverage

This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Blattodea
Family  Blattidae

Temporal Coverage

Start Date / End Date 2005-08-15 / 2019-05-28

Project Data

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Title The InBIO Barcoding Initiative Database: Blattodea 01
Identifier InBIO Barcoding Initiative
Funding InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Study Area Description Continental Portugal
Design Description Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%).

The personnel involved in the project:

Author
Horst Bohn
Originator
Pedro Sousa

Sampling Methods

The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.

Study Extent Continental Portugal.
Quality Control All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).

Method step description:

  1. Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
  2. Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
  3. Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
  4. DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
  5. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

Collection Data

Collection Name InBIO Barcoding Initiative
Collection Identifier 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Parent Collection Identifier CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Specimen preservation methods Alcohol
Curatorial Units Between 1 and 38 Voucher tube ,  Between 1 and 28 DNA extractions

Additional Metadata

Purpose The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Alternative Identifiers 64704ea2-6caf-40fd-a323-641b536f5152
http://ipt.gbif.pt/ipt/resource?r=blattodea_1