The InBIO Barcoding Initiative Database: Blattodea 01

Occurrence
最新版本 published by CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal on 12月 14, 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
首頁:
http://www.boldsystems.org
發布日期:
2020年12月14日
授權條款:
CC-BY 4.0

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說明

The dataset contains 38 records of Blattodea collected from 2005 to 2019 in continental Portugal. Specimens were identified to species level, except for a single record identified to genus level. Of the eight species in the dataset, seven are cockroaches and one is a termite. Specimens were detected during fieldwork by direct search and stored in 96% ethanol. A tissue sample (one leg), was collected from each individual, from which DNA was extracted. The DNA barcoding of these specimens was conducted within the InBIO Barcoding Initiative (IBI), funded by EnvMetaGen and PORBIOTA projects. DNA sequences were deposited in the Barcode of Life Data System (BOLD) and GenBank databases. Preserved specimens and associated DNA extracts are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources).

資料紀錄

此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 38 筆紀錄。

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Ferreira S, Bohn H, Sousa P, Beja P (2020). The InBIO Barcoding Initiative Database: Blattodea 01. Version 1.1. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset. https://doi.org/10.15468/m7f8sg

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 64704ea2-6caf-40fd-a323-641b536f5152。  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。

關鍵字

Blattodea; Cockroach; Termite; Species; Cytochrome c oxidase I (COI); Occurrence; DNA barcode; Continental Portugal

聯絡資訊

Sónia Ferreira
  • 元數據提供者 ●
  • 出處 ●
  • 連絡人
  • Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
  • Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
  • +351 252660411
Horst Bohn
  • 元數據提供者 ●
  • 出處
  • Professor
Zoologische Staatssammlung München
  • Staatliche Naturwissenschaftliche Sammlungen Bayerns (SNSB), Zoologische Staatssammlung München, Münchhausenstraße 21
81247 München Munich
Bavaria
DE
  • +49 89 8107144
Pedro Sousa
  • 元數據提供者 ●
  • 出處
  • Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
  • Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
  • +351 252660411
Pedro Beja
  • 元數據提供者 ●
  • 出處 ●
  • 連絡人
  • Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto
  • Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7
4485-661 Vairão
Porto
PT
  • +351 252660411

地理涵蓋範圍

Continental Portugal.

界定座標範圍 緯度南界 經度西界 [37.176, -8.917], 緯度北界 經度東界 [41.892, -6.561]

分類群涵蓋範圍

This dataset is composed of data relating to 38 Blattodea specimens, 37 of which were determined to species level, and one to genus level. Overall, eight species are represented in the dataset, belonging to seven genera and 3 families.

Kingdom Animalia
Phylum Arthropoda
Class Insecta
Order Blattodea
Family Blattidae

時間涵蓋範圍

起始日期 / 結束日期 2005-08-15 / 2019-05-28

計畫資料

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

計畫名稱 The InBIO Barcoding Initiative Database: Blattodea 01
辨識碼 InBIO Barcoding Initiative
經費來源 InBIO Barcoding Initiative was funded by the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No 668981 and by the project PORBIOTA - Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the project “Promoção dos serviços de ecossistemas no Parque Natural Regional do Vale do Tua: Controlo de Pragas Agrícolas e Florestais por Morcegos” funded by the Agência de Desenvolvimento Regional do Vale do Tua, and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
研究區域描述 Continental Portugal
研究設計描述 Specimens in this dataset were collected in field work carried out between 2005 and 2019. The vast majority of the specimens were morphologically identified (80%) and DNA barcoded (75%).

參與計畫的人員:

Pedro Beja
  • 作者
Sónia Ferreira
  • 作者
Horst Bohn
  • 作者
Pedro Sousa
  • 出處

取樣方法

The studied material was collected in 23 different localities from continental Portugal. Sampling was conducted between 2005 and 2019, with specimens collected by direct search during fieldwork and stored in 96% ethanol for downstream molecular analysis. Morphological identifications were performed using the available literature.

研究範圍 Continental Portugal.
品質控管 All DNA barcodes sequences were compared against the BOLD database and the 99 top hits were inspected in order to detect possible issues due to contaminations or misidentifications. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org).

方法步驟描述:

  1. Specimens were collected in 23 different localities of continental Portugal. Field work was carried out between 2005 and 2019, with 84% of the records made in the interval between 2015 to 2019.
  2. Specimens were collected during fieldwork by direct search and preserved in 96% alcohol. Captured specimens were deposited in the IBI reference collection at CIBIO (Research Center in Biodiversity and Genetic Resources).
  3. Specimens were morphologically identified with the assistance of a binocular stereoscopic microscope and using the available literature.
  4. DNA barcodes were sequenced from a subset of samples from each species in the dataset, selected to represent the best geographical coverage available for the species, for a total of 28 samples. For this, one leg was removed from each individual, DNA was then extracted, and a 658 bp COI DNA barcode fragment was amplified and sequenced. For two specimens of Reticulitermes grassei only a 325 bp fragment was sequenced. DNA extracts were deposited in the IBI collection.
  5. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  6. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.3 (http://openrefine.org/).

收藏資料

蒐藏名稱 InBIO Barcoding Initiative
蒐藏編號 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
上層採集品識別碼 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
標本保存方法 Alcohol
管理單位 在...之間 1 和 38 Voucher tube ,  在...之間 1 和 28 DNA extractions

額外的詮釋資料

目的

The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.

替代的識別碼 64704ea2-6caf-40fd-a323-641b536f5152
http://ipt.gbif.pt/ipt/resource?r=blattodea_1