Descripción
The dataset contains 8 records of Snakeflies (Raphidioptera) species collected from 2016 to 2019 in continental Portugal. In total, three of the six species known to occur in Portugal are represented in the dataset. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 8 registros.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera). v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/p7xzf3
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Esta obra está bajo una licencia Creative Commons de Atribución/Reconocimiento (CC-BY 4.0).
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 8129bd30-6a5e-4d72-b602-75355ac73182. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF Portugal.
Palabras clave
Raphidioptera; Raphidiidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
Contactos
- Proveedor De Los Metadatos ●
- Originador
http://orcid.org/0000-0002-8733-4104
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
- Proveedor De Los Metadatos
- Proveedor De Los Metadatos ●
- Originador ●
- Punto De Contacto
- Proveedor De Los Metadatos
Cobertura geográfica
Continental Portugal.
| Coordenadas límite | Latitud Mínima Longitud Mínima [40, -8,2], Latitud Máxima Longitud Máxima [41,5, -6,7] |
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Cobertura taxonómica
This dataset is composed of data relating to 8 Snakefly (Raphidioptera) specimens belonging to three species present in Portugal. All species belong to the family Raphidiidae.
| Reino | Animalia |
|---|---|
| Filo | Arthropoda |
| Class | Insecta |
| Orden | Raphidioptera |
| Familia | Raphidiidae |
Cobertura temporal
| Fecha Inicial / Fecha Final | 2016-06-01 / 2019-04-20 |
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Datos del proyecto
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| Título | The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera) |
|---|---|
| Identificador | InBIO Barcoding Initiative |
| Fuentes de Financiación | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| Descripción del área de estudio | Specimens were captured during several field incursions from 2016 to 2019 in six localities, five in the district of Bragança and one in the district of Leiria. |
| Descripción del diseño | The specimens in this dataset were collected in field work incursions carried out between 2016 and 2019, encompassing several sites throughout. Specimens of Raphidioptera were detected by direct search. |
Personas asociadas al proyecto:
Métodos de muestreo
Specimens were captured from 2016 to 2019 in continental Portugal by direct search in six sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected male specimens were morphologically identified using a binocular stereoscopic microscope. Females specimens were identified using the BOLD (Barcode of Life Database) Identification System.
| Área de Estudio | Specimens of Raphidioptera were captured in six sites, distributed across two districts of the country. Sampling was conducted from 2016 to 2019, in several field work expeditions. |
|---|---|
| Control de Calidad | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). |
Descripción de la metodología paso a paso:
- Specimens of Raphidioptera were captured in continental Portugal from 2016 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Monserrat and Papenberg, 2015). Females were identified using the BOLD (Barcode of Life Database) Identification System.
- DNA barcodes were obtained for all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
Datos de la colección
| Nombre de la Colección | InBIO Barcoding Initiative |
|---|---|
| Identificador de la Colección | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| Identificador de la Colección Parental | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| Métodos de preservación de los ejemplares | Alcohol |
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| Unidades curatoriales | Entre 1 y 8 Voucher tube , Entre 1 y 8 DNA extractions |
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Referencias bibliográficas
- Monserrat, V. J. and Papenberg, D. (2015). Los rafidiópteros de la Península Ibérica (Insecta, Neuropterida: Raphidioptera). Graellsia, 71 (1). pp. 1-90. doi: 10.3989/graellsia.2015.v71.116 doi: 10.3989/graellsia.2015.v71.116
Metadatos adicionales
| Propósito | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
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| Identificadores alternativos | 8129bd30-6a5e-4d72-b602-75355ac73182 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_raphidioptera |