説明
The dataset contains 8 records of Snakeflies (Raphidioptera) species collected from 2016 to 2019 in continental Portugal. In total, three of the six species known to occur in Portugal are represented in the dataset. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
データ レコード
この オカレンス(観察データと標本) リソース内のデータは、1 つまたは複数のデータ テーブルとして生物多様性データを共有するための標準化された形式であるダーウィン コア アーカイブ (DwC-A) として公開されています。 コア データ テーブルには、8 レコードが含まれています。
この IPT はデータをアーカイブし、データ リポジトリとして機能します。データとリソースのメタデータは、 ダウンロード セクションからダウンロードできます。 バージョン テーブルから公開可能な他のバージョンを閲覧でき、リソースに加えられた変更を知ることができます。
バージョン
次の表は、公にアクセス可能な公開バージョンのリソースのみ表示しています。
引用方法
研究者はこの研究内容を以下のように引用する必要があります。:
Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera). v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/p7xzf3
権利
研究者は権利に関する下記ステートメントを尊重する必要があります。:
パブリッシャーとライセンス保持者権利者は CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF登録
このリソースをはGBIF と登録されており GBIF UUID: 8129bd30-6a5e-4d72-b602-75355ac73182が割り当てられています。 GBIF Portugal によって承認されたデータ パブリッシャーとして GBIF に登録されているCIBIO (Research Center in Biodiversity and Genetic Resources) Portugal が、このリソースをパブリッシュしました。
キーワード
Raphidioptera; Raphidiidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
連絡先
- メタデータ提供者 ●
- 最初のデータ採集者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
- メタデータ提供者
- メタデータ提供者 ●
- 最初のデータ採集者 ●
- 連絡先
- メタデータ提供者
地理的範囲
Continental Portugal.
座標(緯度経度) | 南 西 [40, -8.2], 北 東 [41.5, -6.7] |
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生物分類学的範囲
This dataset is composed of data relating to 8 Snakefly (Raphidioptera) specimens belonging to three species present in Portugal. All species belong to the family Raphidiidae.
Kingdom | Animalia |
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Phylum | Arthropoda |
Class | Insecta |
Order | Raphidioptera |
Family | Raphidiidae |
時間的範囲
開始日 / 終了日 | 2016-06-01 / 2019-04-20 |
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プロジェクトデータ
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
タイトル | The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera) |
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識別子 | InBIO Barcoding Initiative |
ファンデイング | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
Study Area Description | Specimens were captured during several field incursions from 2016 to 2019 in six localities, five in the district of Bragança and one in the district of Leiria. |
研究の意図、目的、背景など(デザイン) | The specimens in this dataset were collected in field work incursions carried out between 2016 and 2019, encompassing several sites throughout. Specimens of Raphidioptera were detected by direct search. |
プロジェクトに携わる要員:
収集方法
Specimens were captured from 2016 to 2019 in continental Portugal by direct search in six sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected male specimens were morphologically identified using a binocular stereoscopic microscope. Females specimens were identified using the BOLD (Barcode of Life Database) Identification System.
Study Extent | Specimens of Raphidioptera were captured in six sites, distributed across two districts of the country. Sampling was conducted from 2016 to 2019, in several field work expeditions. |
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Quality Control | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). |
Method step description:
- Specimens of Raphidioptera were captured in continental Portugal from 2016 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Monserrat and Papenberg, 2015). Females were identified using the BOLD (Barcode of Life Database) Identification System.
- DNA barcodes were obtained for all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
コレクションデータ
コレクション名 | InBIO Barcoding Initiative |
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コレクション識別子 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
Parent Collection Identifier | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
標本保存方法 | Alcohol |
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Curatorialユニット | 範囲 1 ~ 8 Voucher tube , 範囲 1 ~ 8 DNA extractions |
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書誌情報の引用
- Monserrat, V. J. and Papenberg, D. (2015). Los rafidiópteros de la Península Ibérica (Insecta, Neuropterida: Raphidioptera). Graellsia, 71 (1). pp. 1-90. doi: 10.3989/graellsia.2015.v71.116 doi: 10.3989/graellsia.2015.v71.116
追加のメタデータ
目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
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代替識別子 | 8129bd30-6a5e-4d72-b602-75355ac73182 |
http://ipt.gbif.pt/ipt/resource?r=ibi_raphidioptera |