說明
The dataset contains 8 records of Snakeflies (Raphidioptera) species collected from 2016 to 2019 in continental Portugal. In total, three of the six species known to occur in Portugal are represented in the dataset. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 8 筆紀錄。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera). v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/p7xzf3
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 8129bd30-6a5e-4d72-b602-75355ac73182。 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal 發佈此資源，並經由GBIF Portugal同意向GBIF註冊成為資料發佈者。
關鍵字
Raphidioptera; Raphidiidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen
聯絡資訊
- 元數據提供者 ●
- 出處
http://orcid.org/0000-0002-8733-4104
- 元數據提供者 ●
- 出處 ●
- 連絡人
- 元數據提供者
- 元數據提供者 ●
- 出處 ●
- 連絡人
- 元數據提供者
地理涵蓋範圍
Continental Portugal.
| 界定座標範圍 | 緯度南界 經度西界 [40, -8.2], 緯度北界 經度東界 [41.5, -6.7] |
|---|
分類群涵蓋範圍
This dataset is composed of data relating to 8 Snakefly (Raphidioptera) specimens belonging to three species present in Portugal. All species belong to the family Raphidiidae.
| Kingdom | Animalia |
|---|---|
| Phylum | Arthropoda |
| Class | Insecta |
| Order | Raphidioptera |
| Family | Raphidiidae |
時間涵蓋範圍
| 起始日期 / 結束日期 | 2016-06-01 / 2019-04-20 |
|---|
計畫資料
The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.
| 計畫名稱 | The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera) |
|---|---|
| 辨識碼 | InBIO Barcoding Initiative |
| 經費來源 | InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002). |
| 研究區域描述 | Specimens were captured during several field incursions from 2016 to 2019 in six localities, five in the district of Bragança and one in the district of Leiria. |
| 研究設計描述 | The specimens in this dataset were collected in field work incursions carried out between 2016 and 2019, encompassing several sites throughout. Specimens of Raphidioptera were detected by direct search. |
參與計畫的人員:
取樣方法
Specimens were captured from 2016 to 2019 in continental Portugal by direct search in six sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected male specimens were morphologically identified using a binocular stereoscopic microscope. Females specimens were identified using the BOLD (Barcode of Life Database) Identification System.
| 研究範圍 | Specimens of Raphidioptera were captured in six sites, distributed across two districts of the country. Sampling was conducted from 2016 to 2019, in several field work expeditions. |
|---|---|
| 品質控管 | To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/). |
方法步驟描述:
- Specimens of Raphidioptera were captured in continental Portugal from 2016 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
- The specimens were morphologically identified resorting to available literature (Monserrat and Papenberg, 2015). Females were identified using the BOLD (Barcode of Life Database) Identification System.
- DNA barcodes were obtained for all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
- All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
- Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).
收藏資料
| 蒐藏名稱 | InBIO Barcoding Initiative |
|---|---|
| 蒐藏編號 | 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970 |
| 上層採集品識別碼 | CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal |
| 標本保存方法 | Alcohol |
|---|
| 管理單位 | 在...之間 1 和 8 Voucher tube , 在...之間 1 和 8 DNA extractions |
|---|
引用文獻
- Monserrat, V. J. and Papenberg, D. (2015). Los rafidiópteros de la Península Ibérica (Insecta, Neuropterida: Raphidioptera). Graellsia, 71 (1). pp. 1-90. doi: 10.3989/graellsia.2015.v71.116 doi: 10.3989/graellsia.2015.v71.116
額外的詮釋資料
| 目的 | The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies. |
|---|---|
| 替代的識別碼 | 8129bd30-6a5e-4d72-b602-75355ac73182 |
| http://ipt.gbif.pt/ipt/resource?r=ibi_raphidioptera |