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The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera)

Latest version published by CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal on Jul 8, 2020 CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal

The dataset contains 8 records of Snakeflies (Raphidioptera) species collected from 2016 to 2019 in continental Portugal. In total, three of the six species known to occur in Portugal are represented in the dataset. Specimens were detected and captured by direct search of the environment. Captured specimens were preserved in 96% ethanol. From each specimen one leg (tissue sample) was removed to be used for DNA extraction. Preserved specimens and DNA extracts are deposited in the InBIO Barcoding Initiative (IBI) collection at the CIBIO (Research Center in Biodiversity and Genetic Resources). Samples of each species were selected for DNA sequencing based on geographic provenance of the corresponding specimen. Sequencing of the 658 bp DNA barcodes was conducted within the InBIO Barcoding Initiative (IBI) and all DNA sequences were submitted to BOLD (Barcode of Life Data System) and GenBank databases.

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 8 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Downloads

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 8 records in English (7 KB) - Update frequency: as needed
Metadata as an EML file download in English (21 KB)
Metadata as an RTF file download in English (16 KB)

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Oliveira D, Ferreira S, Sousa P, Beja P (2020): The InBIO Barcoding Initiative Database: Portuguese Snakeflies (Raphidioptera). v1.3. CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. Occurrence dataset https://doi.org/10.15468/p7xzf3

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 8129bd30-6a5e-4d72-b602-75355ac73182.  CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Portugal.

Keywords

Raphidioptera; Raphidiidae; Species; Occurrence; Cytochrome c oxidase I (COI); DNA barcode; Continental Portugal; Specimen

Contacts

Who created the resource:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Sousa
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Who can answer questions about the resource:

Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Who filled in the metadata:

Daniel Oliveira
Master’s Student
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto PT
Sónia Ferreira
Post-Doc Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Sousa
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411
Pedro Beja
Coordinating Researcher
CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto Campus Agrário de Vairão, Rua Padre Armando Quintas, nº 7 4485-661 Vairão Porto PT +351 252660411

Geographic Coverage

Continental Portugal.

Bounding Coordinates South West [40, -8.2], North East [41.5, -6.7]

Taxonomic Coverage

This dataset is composed of data relating to 8 Snakefly (Raphidioptera) specimens belonging to three species present in Portugal. All species belong to the family Raphidiidae.

Kingdom  Animalia
Phylum  Arthropoda
Class  Insecta
Order  Raphidioptera
Family  Raphidiidae

Temporal Coverage

Start Date / End Date 2016-06-01 / 2019-04-20

Project Data

The InBIO Barcoding Initiative aims to develop a reference collection of DNA barcoding sequences covering Portuguese invertebrate taxa. Within the project, a special focus is afforded to insects, given their relevance to food webs, ecosystems functioning, high diversity and limited number of DNA barcoding sequences of interest.

Title The InBIO Barcoding Initiative Database: Portuguese Stick Insects (Phasmatodea)
Identifier InBIO Barcoding Initiative
Funding InBIO Barcoding Initiative is funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No 668981 and by the project PORBIOTA — Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), supported by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). The fieldwork benefited from EDP Biodiversity Chair, the FCT project PTDC/ASP-SIL/30250/2017 - POCI-01-0145-FEDER-030250 and includes research conducted at the Long Term Research Site of Baixo Sabor (LTER_EU_PT_002).
Study Area Description Specimens were captured during several field incursions from 2016 to 2019 in six localities, five in the district of Bragança and one in the district of Leiria.
Design Description The specimens in this dataset were collected in field work incursions carried out between 2016 and 2019, encompassing several sites throughout. Specimens of Raphidioptera were detected by direct search.

The personnel involved in the project:

Sampling Methods

Specimens were captured from 2016 to 2019 in continental Portugal by direct search in six sampling sites. Captured insects were preserved in 96% ethanol and stored at the InBIO Barcoding Initiative reference collection for downstream molecular analysis. Collected male specimens were morphologically identified using a binocular stereoscopic microscope. Females specimens were identified using the BOLD (Barcode of Life Database) Identification System.

Study Extent Specimens of Raphidioptera were captured in six sites, distributed across two districts of the country. Sampling was conducted from 2016 to 2019, in several field work expeditions.
Quality Control To each specimen the morphological identification was contrasted with the results of the Blast of the newly generated DNA barcodes in BOLD Identification Engine. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Method step description:

  1. Specimens of Raphidioptera were captured in continental Portugal from 2016 and 2019 by direct search of the sampling site. Collected specimens were preserved in 96% ethanol prior to storage.
  2. The specimens were morphologically identified resorting to available literature (Monserrat and Papenberg, 2015). Females were identified using the BOLD (Barcode of Life Database) Identification System.
  3. DNA barcodes were obtained for all specimens. For this, one leg was removed from each individual from which a 658 bp COI DNA barcode fragment was extracted, amplified and sequenced.
  4. All obtained sequences were submitted to BOLD and GenBank databases and to each sequenced specimen the morphological identification was contrasted with the results of the BLAST of the newly generated DNA barcodes in BOLD Identification Engine.
  5. Prior submission to GBIF, data was checked for errors and inconsistencies with OpenRefine 3.2 (http://openrefine.org/).

Collection Data

Collection Name InBIO Barcoding Initiative
Collection Identifier 4ec2b246-f5fa-4b90-9a8d-ddafc2a3f970
Parent Collection Identifier CIBIO (Research Center in Biodiversity and Genetic Resources) Portugal
Specimen preservation methods Alcohol
Curatorial Units Between 1 and 8 Voucher tube ,  Between 1 and 8 DNA extractions

Bibliographic Citations

  1. Monserrat, V. J. and Papenberg, D. (2015). Los rafidiópteros de la Península Ibérica (Insecta, Neuropterida: Raphidioptera). Graellsia, 71 (1). pp. 1-90. doi: 10.3989/graellsia.2015.v71.116 doi: 10.3989/graellsia.2015.v71.116

Additional Metadata

Purpose The InBIO Barcoding Initiative (IBI) aims to develop a reference collection of DNA barcoding sequences primarily covering Portuguese invertebrate taxa providing a tool in ecological studies.
Alternative Identifiers 8129bd30-6a5e-4d72-b602-75355ac73182
http://ipt.gbif.pt/ipt/resource?r=ibi_raphidioptera